miR-21诱导FOXO1调控前列腺癌细胞EMT及侵袭转移  

MiR-21 promotes the EMT and invasion of prostate cancer cell by inducing FOXO1

作　　者：陈颖虎 王震霆[3] 钟朝晖 CHEN Ying- hu;WANG Zhenting;ZHONG Chaohui(Department Urology Department of Xiangya Second hospital, Cen- tral South University, Changsha 410000, China)

机构地区：[1]中南大学湘雅二医院泌尿外科,长沙410399 [2]解放军第四二五医院外一科 [3]海口市人民医院泌尿外科

出　　处：《临床外科杂志》2018年第3期223-225,共3页Journal of Clinical Surgery

摘　　要：目的探讨miR-21诱导FOXO1调控前列腺癌细胞EMT及侵袭转移。方法人工合成miR-21 mimic/inhibitor及其对照组序列,分别转入前列腺癌细胞株C4-2、DU145中,收集细胞行RT-qPCR检测miR-21、FOXO1 mRNA表达,行Western blot检测FOXO1、E-cadherin和N-cadherin表达,行Transwell试验检测转染后前列腺癌细胞侵袭透膜能力变化。结果前列腺癌细胞株C4-2、DU145转染miR-21 mimic/inhibitor及对照序列后,RT-qPCR检测miR-21表达升高/降低明显(P<0.05),RT-qPCR检测FOXO1 mRNA表达与miR-21一致。在Western blot结果中,转染miR-21 mimic前列腺癌细胞FOXO1、N-cadherin表达高于对照组,E-cadherin表达相反;转染miR-21 inhibitor前列腺癌细胞FOXO1、N-cadherin表达低于对照组,E-cadherin表达相反。Transwell试验显示,转染miR-21 mimic前列腺癌细胞侵袭透膜能力较对照组增强(P<0.01),转染miR-21 inhibitor前列腺癌细胞侵袭能力较对照组减弱(P<0.01)。结论 miR-21通过诱导FOXO1调控前列腺癌细胞EMT及侵袭转移。Objective To investigate the mechanism of EMT and invasion promoted by miR-21 in prostate cancer cells.Methods The sequence of miR-21 mimic/inhibitor was firstly designed and syn-thesized.Then miR-21 mimic/inhibitor and its control were transfected into prostate cancer cells CA-2 and DUI45,respectively.And cells were collected for mRNA isolation and RT-qPCR analysis for miR-21 and FOXO1.FOXO1,E-eadherin and N-cadherin were detected by Western blot,and the invasion of prostate cancer cells were detected by transweil assays.Results The expression of miR-21 increased in both C4-2 and DU145 after transfection,and the expression of FOXO1 mRNA increased at the same time（P 〈 0.01）.The expression of miR-21 and FOXO1 mRNA in C4-2 and DU145 was decreased by miR-21 in-hibitor（P 〈 0.05）.The protein expression of FOXO1 and N-cadherin in CA-2 and DU145 increased after the treatment of miR-21,while that of E-cadherin decreased.The protein expression of FOXO1 and N-cad-herin in C4-2 and DU145 decreased after the treatment of miR-21 inhibitor,while that of E-cadherin in-creased.The invasive level in C4-2 and DU145 increased after the treatment of miR-21,while that de-creased after the treatment of miR-21 inhibitor.Conclusion MiR-21 promotes EMT and invasion by in-ducing FOXO1 in prostate cancer ceils.

关 键 词：前列腺癌细胞 FOXO1 miRNA-21EMT 

分 类 号：R737.25[医药卫生—肿瘤]