磁性固相萃取-高效液相色谱法测定葡萄酒中罗丹明B的含量  

Magnetic Solid Phase Extraction-HPLC Determination of Rhodamine B in Wine

作　　者：王露[1] 徐瑞[1] 王芹[1] 宋鑫[1] 冯晓青[1] 汪怡 杭学宇[1] WANG Lu;XU Rui;WANG Qin;SONG Xin;FENG Xiaoqing;WANG Yi;HANG Xueyu(Huai＇an Center for Disease Control and Prevention, Huai＇an 223001, China)

机构地区：[1]淮安市疾病预防控制中心,淮安223001

出　　处：《理化检验（化学分册）》2018年第4期403-407,共5页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：江苏省卫生厅预防医学科研基金(Y2015035);淮安市科技局科技支撑(社会发展)项目(HAS2015030)

摘　　要：样品溶液(pH 4.0,10 mL)以流量1.0 mL·min^(-1)经自制磁性石墨烯(CoFe_2O_4-G)20mg萃取,用1mL丙酮洗脱。所得溶液中的罗丹明B采用高效液相色谱法进行测定。罗丹明B的质量浓度在0.50~10μg·L^(-1)内与其峰面积呈线性关系,检出限(3S/N)为0.08μg·L^(-1)。按标准加入法进行回收试验,回收率为99.0%~101%,相对标准偏差(n=11)为1.6%~4.0%。The sample solution（pH 4.0,10 mL） was extracted with homemade magnetic graphene（CoFe2O4-G） of 20 mg at the flow rate of 1.0 mL·min^-1,and acetone of 1 mL was used as the eluent.Rhodamine B in the above solution was determined by HPLC.Linear relationship between peak area and mass concentration of rhodamine B was found in the range of 0.50^-10μg·L^-1,with detection limit（3 S/N） of 0.08μg·L^-1.Test for recovery was made by standard addition method,giving results in the range of 99.0%^-101%,with RSDs（n=11）ranged from 1.6%to 4.0%.

关 键 词：磁性固相萃取 高效液相色谱法 CoFe2O4-石墨烯 罗丹明B 葡萄酒 

分 类 号：O652.63[理学—分析化学]