小鼠颅脑爆震伤模型制作及大脑皮层早期病理变化  

Mouse model of blast traumatic brain injury and early pathological changes in cerebral cortex

作　　者：扶宇 王本瀚[1] 初霞[1] 李经纶[1] 曹付强[1] 刘雷震[1] 姚安会[1] Fu Yu;Wang Benhan;Chu Xia;Li Jinglun;Cao Fuqiang;Liu Leizhen;Yao Anhui(The 153th Center Hospital of PIA, Zhengzhou, 450042;Xinxiang Medical University, Xinxiang 453003, China)

机构地区：[1]解放军第153中心医院,郑州450042 [2]新乡医学院,新乡453003

出　　处：《神经解剖学杂志》2018年第2期249-254,共6页Chinese Journal of Neuroanatomy

基　　金：全军医学科技青年课题(14QNP029)

摘　　要：目的:建立小鼠颅脑爆震伤模型研究其早期大脑皮层病理变化。方法:60只雄性C57BL/6小鼠随机分为对照组、爆震伤组。小鼠麻醉后,置于距离爆炸源40 cm处,爆炸源为纸质外壳球形5 g TNT炸药,通过电子引爆线引爆,制作小鼠颅脑爆震伤模型。爆震伤后观察动物的存活率及神经功能变化,并在损伤后不同时间点(6 h、1 d、3 d、5 d、7 d)取材,通过H.E.染色的方法观察小鼠爆震伤后大脑皮层早期的病理变化:淋巴细胞浸润、嗜酸样变性、空泡样变性,通过尼氏染色研究小鼠爆震伤后大脑皮层神经元形态的变化。结果:5 g TNT爆震组存活率下降(65.0%±3.2%);爆震伤后早期可见淋巴细胞浸润,统计显示在爆震伤后6 h明显升高(21.5%±4.0%),并维持至损伤后3 d(20.4%±3.9%),随后5~7 d有所下降。H.E.染色结果显示,与对照组(3.3%±1.3%)相比,爆震伤组嗜酸样变性细胞数在伤后6 h明显增加(23.5%±5.4%),持续至损伤后3 d(24.9%±4.4%),至损伤后5 d(16.3%±4.4%)、7 d(16.3%±5.2%)有所下降,但仍高于对照组。而对于空泡样变性,与对照组(6.9%±5.2%)相比,在爆震伤后6 h明显升高(30.6%±12.3%),至爆震伤后3 d进一步升高(37.5%±7.7%),并继续升高至损伤后7 d(44.7%±17.9%)。尼氏染色发现爆震伤后随着时间的延长,神经元胞体水肿,空泡变性增多,尼氏小体染色变浅且数目逐渐减少。结论:5 g TNT爆震伤可模拟冲击波原发效应,根据不同时间点病理变化,说明5 g TNT小鼠爆震伤可以用来模拟研究颅脑爆震伤。Object： To establish a model of blast traumatic brain injury in mice and study its early pathological changes in cerebral cortex. Methods： 60 male C57 BL/6 mice were randomly divided into two groups： control group and blast brain traumatic injury group（ b TBI）. After the mice were anesthetized,they were placed at 40 cm from the explosion source. The explosive source was a paper shell contained 5 g TNT. The model of b TBI was made by exploding the explosive source through an electronic detonating cord. After blast injury,the changes of survival rate and neuronal function were recorded. At different time points after injury（ 6 h,1 d,3 d,5 d,7 d）,early pathological changes in mice were observed by H. E. staining method,including： lymphocytic infiltration,eosinophilic degeneration,vacuolar degeneration. The morphological changes of cortical neurons in the cerebral cortex after b TBI was studied by Nissl staining. Results： The survival rate decreased in 5 g TNT group（ 65 ± 3. 2%） after b TBI. There was lymphocyte infiltra-tion at early time after blast injury,and statistics showed that it increased significantly at 6 h（ 21. 5 ± 4. 0%） after blast injury,and maintained to 3 d（ 20. 4 ± 3. 9%）,and then decreased at 5 ~ 7 d post injury. H. E. staining showed that,compared with the control group（ 3. 3 ± 1. 3%）,the number of acidophilic degeneration cells was significantly increased（ 23. 5 ± 5. 4%） at 6 h after blast injury,and maintained to 3 d（ 24. 9 ± 4. 4%） after injury,and decreased at 5 d（ 16. 3 ± 4. 4%） and 7 d（ 16. 3 ± 5. 2%） after injury,which were still higher than the control group. As for vacuolar degeneration,it was significantly increased at 6 h in the blast injury group（ 30. 6 ± 12. 3%） than the control group（ 6. 9 ± 5. 2%）,and further increased at 3 d（ 37. 5 ± 7. 7%） after blast injury,and continued to rise until 7 d（ 44. 7 ± 17. 9%） post injury. Nissl staining showed that after the blast injury,the neurons body edema and vacuolar

关 键 词：爆震伤 颅脑损伤 小鼠模型 病理变化 

分 类 号：R-332[医药卫生] R651.15