竹节参皂苷对D-半乳糖致SH-SY5Y神经细胞损伤的保护作用研究  

Study on the protective effect of Panax japonicus saponins on D-galactose induced SH-SY5Y neuron injury

作　　者：万静枝 袁丁[1] 王婷[2] Wan Jingzhi;Yuan Ding;Wang Ting(Affiliated Renhe Hospital,China Three Gorges University;Medical College of China Three Gorges Universit;Yichang 443002,Hubei,China)

机构地区：[1]三峡大学附属仁和医院,湖北宜昌443001 [2]三峡大学医学院,湖北宜昌443002

出　　处：《贵州医药》2018年第4期387-389,共3页Guizhou Medical Journal

基　　金：国家自然科学基金项目(编号:81374001)

摘　　要：目的研究竹节参皂苷保护D-半乳糖致SH-SY5Y神经细胞损伤的作用及机制。方法采用50g/L的D-半乳糖刺激SH-SY5Y神经细胞,以不同浓度竹节参皂苷(1,5,25μg/mL)和50g/L的D-半乳糖共同孵育SHSY5Y神经细胞24h。JC-1试剂盒检测细胞线粒体膜电位,Western blot检测细胞Sirt1蛋白的表达。结果竹节参皂苷能显著升高线粒体膜电位和Sirt1蛋白的表达。结论竹节参皂苷通过激活Sirt1抑制线粒体功能障碍从而改善D-半乳糖对SH-SY5Y神经细胞造成的损伤。Objective To observe the protective effect and mechanism of Panax japonicus saponins on D-galactose induced SH-SY5 Yneuron injury.Methods The 50 g/L D-galactose was used to stimulate SH-SY5 Yneuron,Different concentrations Panax japonicus saponins（1,5,25μg/mL）and D-galactose co-incubated with SH-SY5 Yneuron for24 h.The changes of mitochondrial membrane potential were identified by JC-1 detection.Protein level of silentmating type information regulation 2 Homolog-1（Sirt1）was detected by Western blot analysis.Results Panax japonicus saponins significantly improved mitochondrial transmembrane potential and the expression of Sirt1 protein.Conclusion Panax japonicus saponins inhibits mitochondrial dysfunction by activating Sirt1,thereby improving D-galactose induced damage to SH-SY5 Yneuron.

关 键 词：竹节神皂苷 SH-SY5Y神经细胞 线粒体膜电位 SIRT1 

分 类 号：R-33[医药卫生] R392.12