利拉鲁肽对匹罗卡品致痫大鼠海马各区神经元凋亡的影响  

Effects of liraglutide on neuronal apoptosis in each zone of hippocampus in rats with epilepsy induced by pilocarpine

作　　者：王瑞芳 薛国芳[2] 连霞[2] 高慧中[2] 曹丽君 郑辑英[2] WANG Ruifang;XUE Guofang;LIAN Xia;GAO Huizhong;CAO Lijun;ZHENG Jiying(Shanxi Medical University,Taiyuan 030000,China;Department of Neurology,Shanxi Medical University Second Hospital,Taiyuan 030000,China)

机构地区：[1]山西医科大学 [2]山西医科大学第二医院神经内科,山西太原030000

出　　处：《中国现代医生》2018年第10期32-36,169,共6页China Modern Doctor

基　　金：中国抗癫痫协会癫痫科研基金-UCB基金(2017007);国家自然科学基金(81601038);山西省科技厅青年科技研究基金(2014021038-4);山西医科大学校创新基金(057602)

摘　　要：目的探讨利拉鲁肽对大鼠癫痫持续状态后海马各区神经元凋亡的影响。方法将雄性SD大鼠(n=54)随机分为空白对照组(n=6)、匹罗卡品模型组(SE组,n=24)、利拉鲁肽干预组(Liraglutide组,n=24);并根据发作终止后的时间点(12 h,1 d,3 d,7 d)将SE组和Liraglutide组各分为4个亚组(n=6)。采用免疫组化技术检测海马CA3和DG区BCL2和BAX蛋白的表达。结果与SE组相比,Liraglutide组在SE后12 h、1 d、3 d时CA3区BCL2表达水平升高(P<0.05),而在SE后7 d时BCL2水平与SE组无差异(P>0.05);在DG区,Liraglutide组在SE后1 d BCL2表达升高(P<0.05),在SE后3 d时表达无差异(P>0.05)。Liraglutide组相较SE组,在SE后12 h,DG区BAX表达开始明显降低(P<0.01);在SE后1 d,CA3区BAX表达降低(P<0.01);且在SE后3 d,Liraglutide组DG区和CA3区BAX表达都高于空白对照组(P<0.05)。结论利拉鲁肽通过抑制癫痫持续状态后海马CA3区和DG区神经元凋亡发挥神经保护作用。Objective To investigate the effect of liraglutide on neuronal apoptosis in each zone of hippocampus after persistent state of epilepsy in rats. Methods Male SD rats（n=54） were randomly divided into blank control group（n=6）,pilocarpine model group（SE group, n=24）, and liraglutide intervention group（Liraglutide group, n=24）; the SE group and liraglutide group were divided into 4 subgroups（n=6） according to the time point after the termination of attack（12 h,1 d, 3 d, 7 d）. Immunohistochemistry was used to detect the expression of BCL2 and BAX proteins in CA3 and DG zones of hippocampus. Results In the liraglutide group, compared with the SE group, the expression of BCL2 in CA3 zone was increased at 12 hours, 1 day and 3 days after SE（P〈0.05）. However, there was no difference in BCL2 level compared with SE group at 7 days after SE（P〈0.05）;in the DG zone, BCL2 expression was increased in liraglutide group one day after SE（P〈0.05）;there was no difference in expression at 3 days after SE（P〉0.05）. In the liraglutide group,the expression of BAX was significantly decreased in DG zone at 12 hours after SE compared with SE group（P〈0.01）;one day after SE, the expression of BAX was decreased in CA3 zone（P〈0.01）;and 3 days after SE, BAX expression in DG zone and CA3 zone in liraglutide group was higher than that in the blank control group（P〈0.05）. Conclusion Liraglutide exerts neuroprotective effect by inhibiting apoptosis of neurons in CA3 and DG zones of hippocampus after persistent states of epilepsy.

关 键 词：GLP-1 癫痫 海马 凋亡 

分 类 号：R742.1[医药卫生—神经病学与精神病学]