miRNA-141对胃癌细胞生长的抑制调节作用  

miRNA-141 negatively regulates gastric cancer cells in vitro

作　　者：刘棣[1] 宋玲琴[1] 张寅斌[1] 闫婉君 赵小瑶 盛倩文 许朋 代志军[1] 康华峰[1] Liu Di;Song Lingqin;Zhang Yinbin;Yan Wanjun;Zhao Xiaoyao;Sheng Qianwen;Xu Peng;Dai Zhijun;Kang Huafeng(Department of Oncology, the Second Affiliated Hospital of Xi＇an Jiaotong University, Shaanxi Xi＇an 710004, China.)

机构地区：[1]西安交通大学第二附属医院肿瘤科,陕西西安710004

出　　处：《现代肿瘤医学》2018年第9期1344-1347,共4页Journal of Modern Oncology

基　　金：中央高校基本科研业务费专项资金资助(编号:2014gjhz11);陕西省科技厅重点研发计划一般项目资助(编号:2017SF-172);必康基金(编号:2017BIKANGJIJIN-020);西安交通大学第二附属医院院青年基金[编号:YJ(QN)201305]

摘　　要：目的:研究miRNA-141在胃癌细胞中的生物学功能。方法:定量PCR检测人胃黏膜细胞GES-1,胃癌细胞株BGC823、MGC803、SGC7901中miRNA-141的表达,通过miRNA-141 inhibitor、mimics分别抑制及促进miRNA-141的表达,观察癌细胞增殖、克隆形成、侵袭能力的变化。结果:胃癌细胞BGC823、MGC803、SGC7901中miRNA-141处于低表达状态。上调miRNA-141的表达后,miRNA-141明显抑制SGC7901细胞增殖(P<0.05),抑制肿瘤细胞克隆形成(P<0.05)及癌细胞侵袭力(P<0.05)。结论:miRNA-141对胃癌细胞SGC7901具有负性调控作用,可以作为潜在的治疗靶点。Objective： To identify the regulation of miRNA-141 on proliferation of gastric cancer cells. Methods：The expression of miRNA-141 in gastric cancer cell line BGC823,MGC803,SGC7901 were detected by Real-time PCR. The MTT was used to detect SGC7901 cell proliferation after miRNA-141 downregulated and upregulated. The colony forming efficiency of miRNA-141 was detected by colony forming assay. The transwell chamber was used to investigate the influence of miRNA-141 on invasion of SGC7901 cells. Results： miRNA-141 were in downregulated statues in BGC823,MGC803,SGC7901 cell lines. The upregulated miRNA-141 could inhibit proliferation,colony forming and cell invasion（ P＜0. 05）. Also the downregulated miRNA-141 promoted cell proliferation,colony forming and cell invasion（ P＜0. 05）. Conclusion： miRNA-141 negatively regulate gastric cancer cell SGC7901,it is a potential therapy target.

关 键 词：MIRNA 胃癌 miRNA-141 

分 类 号：R735.2[医药卫生—肿瘤]