Beclin基因表达改变对上皮性卵巢癌细胞顺铂敏感性的影响及机制探讨  

Effects of Variant Beclin Gene Expression on Cisplatin Sensitivity in Epithelial Ovarian Cancer Cells and Its Mechanism

作　　者：杨飏 莫生娣 罗嘉 侯世敏 徐静 YANG Yang;MO Shengdi;LUO Jia;HOU Shimin;XU Jing(Obstetrics and Gynecology Department, Xiangxi People’ s Hospital, Jishou, Hunan, 416000, China)

机构地区：[1]湘西自治州人民医院妇产科,湖南吉首416000

出　　处：《肿瘤药学》2018年第2期247-250,共4页Anti-Tumor Pharmacy

摘　　要：目的探讨Beclin基因表达改变对上皮性卵巢癌SKOV3细胞顺铂敏感性的影响,分析其与肿瘤细胞自噬的关系。方法用SKOV3细胞系作为研究载体,通过RT-PCR检测比较SKOV3细胞株与SKOV3顺铂耐药细胞株(SKOV3/DDP)中Beclin基因表达的差异。通过siRNA干扰SKOV3、SKOV3/DDP细胞中Beclin目的基因的表达,采用MTT法比较细胞对顺铂的敏感性。使用自噬抑制剂3-甲基腺嘌呤(3-MA)干预SKOV3/DDP细胞,采用RT-PCR检测Beclin基因的表达,MTT法检测细胞对顺铂的敏感性。结果 SKOV3耐药株Beclin基因表达明显高于SKOV3细胞,差异有统计学意义(P<0.05)。siRNA干预目的基因Beclin表达后,顺铂对SKOV3、SKOV3/DDP、SKOV3si-Beclin、SKOV3/DDPsi-Beclin的半数抑制浓度(IC50)分别为(12.36±2.31)μg·mL^(-1)、(17.31±1.91)μg·mL^(-1)、(7.57±2.05)μg·mL^(-1)、(12.46±1.58)μg·mL^(-1),与干预前比较,差异有统计学意义(P<0.05)。3-MA可抑制SKOV3/DDP细胞Beclin表达,并增加细胞对顺铂的敏感性,差异有统计学意义(P<0.05)。结论 Beclin基因表达改变与SKOV3细胞对顺铂的敏感性有着密切的关联,且这一关联与自噬现象有关。Objective To explore the effect of variant Beclin gene expression on cisplatin sensitivity of epithelial ovarian cancer cells and its mechanism. Methods The Beclin expression was detected in SKOV3 and SKOV3/DDP cells via RT-PCR. siRNA was used to in-hibit the Beclin expression in SKOV3 and SKOV3/DDP. The autophagy inhibitor 3-methyladenine （3-MA） was applied to interfere with the SKOV3/DDP cells. MTT method was applied to detect the sensitivity of cells to cisplatin. Results The expression of Beclin gene in SKOV3 resistant strains was significantly higher than in SKOV3 cells, and the difference was statistically significant （P〈0.05）. After siRNA inhibi-tion, the IC50 of SKOV3, SKOV3/DDP, SKOV3si-Beclin and SKOV3/DDPsi-Beclin were respectively （12.36±2.31） μg · mL-1, （17.31±1.91） μg · mL-1, （7.57±2.05） μg · mL-1 and （12.46±1.58） μg · mL-1. The difference was statistically significant when compared with before siRNA inhibition （P〈0.05）.3-MA could inhibit the expression of Beclin in SKOV3/DDP cells and increase the sensitivity of cells to cisplatin. Conclusion The variant Beclin expression was directly associated with the sensitivity of SKOV3 cells to cisplatin, and this associationwas related to autophagy.

关 键 词：Beclin基因 上皮性卵巢癌 顺铂 敏感性 

分 类 号：R737.31[医药卫生—肿瘤]