^18F-氟赤式硝基咪唑microPET／CT显像评估马蔺子素对乳腺癌的放疗增敏作用  

Evaluation of radiosensitivity enhancement by irisquinone with ^18F-FETNIM microPET/CT imaging in breast cancer bearing nude mice

作　　者：谯凤 徐慧琴[1] 张丹[1] 苏晓雨 汪会[1] 余文静 Qiao Feng;Xu Huiqin;Zhang Dan;Su Xiaoyu;Wang Hui;Yu Wenjing(Department of Nuclear Medicine, the First Affiliated Hospital of Anhui Medical University, Hefei 230022, Chin)

机构地区：[1]安徽医科大学第一附属医院核医学科,合肥230022

出　　处：《中华核医学与分子影像杂志》2018年第4期262-265,共4页Chinese Journal of Nuclear Medicine and Molecular Imaging

基　　金：国家自然科学基金（81371587）

摘　　要：目的探讨^18F－氟赤式硝基咪唑（FETNIM） microPET/CT显像评估马蔺子素（IR）对乳腺癌放疗的增敏作用。方法将32只荷MDA－MB－231乳腺癌裸鼠按随机数字表法分为单纯放疗组、IR＋放疗组、IR组和对照组（每组8只），分别在放疗前和放疗后24 h行^18F－FETNIM microPET/CT显像，观察4组荷瘤裸鼠肿瘤部位最大标准摄取值（SUVmax）变化。观察肿瘤组织病理改变，用免疫组织化学法分析乏氧诱导因子－1α（HIF－1α）的表达情况。采用配对t检验、单因素方差分析、最小显著差异t检验和Pearson相关分析处理数据。结果放疗前，单纯放疗组、IR＋放疗组、IR组和对照组SUVmax差异无统计学意义（1.429±0.090、1.430±0.076、1.445±0.071和1.432±0.074；F＝0.072，P〉0.05）。放疗后24 h，单纯放疗组和IR＋放疗组的SUVmax下降（1.075±0.098和0.890±0.076；t值：12.888和33.217，均P〈0.05），IR＋放疗组下降更明显（t＝4.197，P〈0.05）；IR组及对照组的SUVmax上升（1.617±0.090和1.644±0.063；t值：－11.009和－16.061，均P〈0.05）。病理结果示，单纯放疗组及IR＋放疗组肿瘤细胞数量减少且坏死区明显，且后者更甚。免疫组织化学结果显示，HIF－1α在IR＋放疗组中表达最低[（26.75±7.19）%; F＝46.745，t值：2.898～9.743，均P〈0.05]。HIF－1α表达与SUVmax呈正相关（r＝0.89，P〈0.05）。结论^18F－FETNIM microPET/CT可以评估IR对荷MDA－MB－231乳腺癌裸鼠肿瘤放疗的增敏作用。ObjectiveTo investigate the effect of irisquinone （IR） on the radiosensitization of MDA-MB-231 breast cancer in nude mice using ^18F-fluoroerythronitroimidazole （18F-FETNIM） microPET/CT.MethodsThirty-two nude mice bearing MDA-MB-231 breast cancer were divided into 4 groups by random number table method （n=8 for each group）： group A was treated with radiotherapy alone, group B was treated with radiotherapy and IR, group C was treated with IR alone, and group D was fed with distilled water. ^18F-FETNIM microPET/CT images were obtained to monitor the maximum standardized uptake value （SUVmax） of tumor before radiotherapy and 24 h after radiotherapy. The mice were sacrificed and tumor tissues were removed for HE staining. The expression of hypoxia inducible factor-1α （HIF-1α） was detected by immunohistochemical （IHC） method. Data were analyzed by paired t test, one-way analysis of variance, the least significant different t test and Pearson correlation analysis.ResultsThere was no significant difference in SUVmax among the 4 groups before radiotherapy （1.429±0.090, 1.430±0.076, 1.445±0.071, 1.432±0.074; F=0.072, P〉0.05）. At 24 h after radiotherapy, the SUVmax of group A and B decreased significantly （1.075±0.098, 0.890±0.076; t values： 12.888, 33.217, both P〈0.05）, and there was significant difference between group B and group A （t=4.197, P〈0.05）. However, the SUVmax of group C and D increased significantly （1.617±0.090, 1.644±0.063; t values： -11.009, -16.061, both P〈0.05）. Pathological results showed that tumor cells in group A and B were reduced. IHC results showed that the expression of HIF-1α was lower in group B than others （（26.75±7.19）%; F=46.745, t values： 2.898-9.743, all P〈0.05）. The expression of HIF-1α was positively correlated with SUVmax （r=0.89, P〈0.05）.Conclusion18F-FETNIM microPET/CT could evaluate the radiosensitization effect of IR on MDA-MB-231 breast cancer in nude mice.

关 键 词：乳腺肿瘤 辐射增敏药 放射疗法 正电子发射断层显像术 体层摄影术 X线计算机 硝基咪唑类 

分 类 号：R730.55[医药卫生—肿瘤] R737.9[医药卫生—临床医学]