叶黄素对乳腺癌细胞活力的影响  

Effects of lutein on viability of breast cancer cells

作　　者：常景芝[1] 王琛[1] 李宜川[1] 刘青 沈永杰[1] 芦琨[1] 郭亚莉 张善锋 王明臣 CHANG Jing-zhi;WANG Chen;LI Yi-chuan;LIU Qing;SHEN Yong-jie;LU Kun;GUO Ya-li;ZHANG Shan-feng;WANG Ming-chen(Department of Biochemistry and Molecular Biology, Shangqiu Medical College, Shangqiu ,476100, China;Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou ,450001, China)

机构地区：[1]商丘医学高等专科学校生物化学与分子生物学教研室,河南商丘476100 [2]郑州大学基础医学院生物化学与分子生物学系,河南郑州450001

出　　处：《中国病理生理杂志》2018年第5期930-933,共4页Chinese Journal of Pathophysiology

基　　金：2015年河南省重点科技攻关项目(No.152102310235)

摘　　要：目的:探究叶黄素对人乳腺癌T47D细胞活力的影响及其可能的作用机制。方法:将人乳腺癌T47D细胞随机分为对照组和叶黄素(浓度分别为6.25、12.5、25和50 mg/L)干预组,采用MTT法检测叶黄素对T47D细胞活力的影响;RT-qPCR检测核因子E2相关因子2(Nrf2),谷胱甘肽过氧化物酶1(GPx1)及超氧化物歧化酶2(SOD2)的mRNA水平;荧光探针DCFH-DA法检测细胞内活性氧簇(ROS)水平;Western blot检测Nrf2和p65的蛋白表达。结果:MTT结果显示叶黄素抑制人乳腺癌T47D细胞的活力,且呈时间和剂量依赖关系。RT-q PCR结果显示各浓度叶黄素干预组细胞中Nrf2,GPx1及SOD2的mRNA水平均较对照组升高(P<0.05);且叶黄素干预组细胞内ROS水平显著下降(P<0.01)。Western blot结果显示,叶黄素干预48 h后,Nrf2蛋白表达增加(P<0.05),p65蛋白表达降低(P<0.05),呈剂量依赖关系。结论 :叶黄素对乳腺癌细胞活力的抑制作用可能与上调Nrf2的表达、诱导抗氧化酶GPx1及SOD2 mRNA表达和降低氧化应激水平,进而阻断NF-κB信号通路有关。AIM： To investigate the effect of lutein on the viability of breast cancer cells and its possible mechanism. METHODS： The human breast cancer T47D cells were divided into control group and lutein（ 6. 25,12. 5,25,50 mg/L） treatment groups. The effect of lutein on the viability of T47D cells was measured by MTT assay. The mRNA expression of nuclear factor erythroid 2-related factor 2（ Nrf2）,glutathione peroxidase 1（ GPx1） and superoxide dismutase 2（ SOD2） was detected by RT-q PCR. Fluorescent probes DCFH-DA was used to determine the production of reactive oxygen species（ ROS）. The protein expression of Nrf2 and p65 was determined by Western blot. RESULTS： The MTT results showed that lutein inhibited T47 D breast cancer cell viability in a dose-and time-dependent manner. The RT-qPCR results showed that the mRNA levels of Nrf2,GPx1 and SOD2 were higher in lutein treatment groups than those in the control group（ P〈0. 05）,and with the increased concentrations and extension of intervention time of lutein,the relative mRNA levels were all increased. The ROS levels were significantly decreased in the lutein-treated groups（ P〈0. 05）. The results of Western blot demonstrated that the protein expression of Nrf2 was significantly increased（ P〈0. 05）,and p65 protein was decreased（ P〈0. 05） in a dose-dependent manner with lutein treatment for 48 h. CONCLUSION： Lutein significantly inhibits the viability of breast cancer cells,and the inhibition roles may be related to up-regulation of the expression of Nrf2,antioxidant enzymes GPx1 and SOD2 mRNA expression and down-regulation of oxidative stress,thus blocking the NF-κB signaling pathway.

关 键 词：叶黄素 乳腺癌 细胞活力 NF-ΚB信号通路 

分 类 号：R329.25[医药卫生—人体解剖和组织胚胎学] R737.9[医药卫生—基础医学]