液相色谱-原子荧光光谱法测定鱼肉中甲基汞不确定度分析  

Uncertainty analysis for detecting methyl mercury in fish tissue with liquid chromatography-atomic fluorescence spectrometry

作　　者：王艳春[1] 籍术良[1] 杨文英[1] WANG Yan-chun,JI Shu-liang,YANG Wen-ying(Physicochemical Department, Tongzhou District Center for Disease Control and Prevention, Beijing 101100, China)

机构地区：[1]北京市通州区疾病预防控制中心理化科,北京101100

出　　处：《中国医学装备》2018年第5期142-145,共4页China Medical Equipment

摘　　要：目的:建立液相色谱-原子荧光光谱法,测定鱼肉中甲基汞的不确定度评定方法。方法:采用液相色谱-原子荧光光谱法测定鱼肉中甲基汞含量,应用国家质量监督检验检疫总局JJF1059.1-2012测量不确定度评定与表示的理论,分析影响其不确定度的因素来源,对各不确定度因素进行评定,并计算合成不确定度,最终给出测量结果的扩展不确定度。结果:液相色谱-原子荧光光谱法测定鱼肉中甲基汞含量的测量结果(ω)=(817.94±105.02)μg/L(k=2,置信水平为95%)。结论:测量不确定度评定可用于液相色谱-原子荧光光谱法测定鱼肉中甲基汞含量的不确定度分析,使测定结果更加可靠。Objective： To establish a method of liquid chromatography-atomic fluorescence spectrometry（LC-AFS）, and carry out uncertainty analysis of detecting methyl mercury in fish tissue. Methods： LC-AFS method was adopted to determine the content of methyl mercury in fish tissue. And the theory of assessment and expression about uncertainty measurement based on JJF1059.1-2012 of State Administration for Market Regulation was applied to analyze the source of factors that affect its uncertainty. Through evaluated various factors of uncertainty and calculated and combined uncertainty to obtain the extended uncertainty of measurement results. Results： The content of methyl mercury in fish tissue by using LC-AFS wasω=（817.94±105.02）μg/kg（k=2, confidence level was 95%）. Conclusion： The measurement uncertainty assessment can be used in the uncertainty analysis that LC-AFS measure content of methyl mercury in fish tissue. Therefore, the results are more reliable.

关 键 词：液相色谱 原子荧光光谱 甲基汞 测量不确定度 

分 类 号：R155.5[医药卫生—营养与食品卫生学]