膜联蛋白A11对人胃癌细胞株SGC7901氟尿嘧啶耐药性的影响及机制  

Effects and mechanism of Annexin All on human gastric cancer cell line SGC7901 drug resistance to fluorouracil

作　　者：贾楠[1] 李勇[1] 赵一杰 赵群[1] 范立侨[1] 檀碧波[1] 刘羽[1] Jia Nan;Li Yong;Zhao Yijie;Zhao Qun;Fan Liqiao;Tan Bibo;Liu Yu(Department of the Third General Surgery, the Fourth Hospital of Hebei Medical University, Shifiazhuang 050011, China)

机构地区：[1]河北医科大学第四医院外三科,石家庄050011

出　　处：《中华实验外科杂志》2018年第4期610-613,共4页Chinese Journal of Experimental Surgery

摘　　要：目的观察膜联蛋白A11（ANXA11）对人胃癌细胞株SGC7901 5-氟尿嘧啶（5-Fu）耐药性影响。方法检测人胃癌细胞株SCG7901、BGC823的ANXA11表达。将ANXA11-小干扰RNA（siRNA）及阴性对照序列瞬时转染入SGC7901细胞，应用细胞计数试剂盒（CCK-8）法检测转染后各组细胞对不同剂量5-Fu（1、10、20、40、80 μg/ml）的药物敏感性改变。应用流式细胞术检测不同处理组凋亡率，应用定量聚合酶链反应（qPCR）、Western blot检测胸腺嘧啶脱氧核苷合成酶（TS）、B细胞淋巴瘤/白血病-2（bcl-2）和bcl-2相关X蛋白（bax）表达的改变。结果ANXA11 mRNA及蛋白表达量在SGC7901中显著高于BGC823（t＝3.212，P＝0.033；t＝－10.768，P＝0.000）。转染组（转染ANXA11-siRNA）细胞的半数抑制剂量（IC50）及20%抑制剂量（IC20）值明显低于阴性、空白对照组（F＝47.500，P＝0.000；F＝45.523，P＝0.000）。转染组、联合组凋亡率较阴性（转染NS-siRNA）、空白对照组（转染Lipofectamine？2000）显著升高（F＝117.525，P＝0.000），且联合组凋亡率较转染组明显升高（t＝9.004，P＝0.000）。转染组及联合组ANXA11蛋白表达较阴性、空白对照组显著降低（F＝118.403，P＝0.000）。转染组及联合组bax的表达上调（F＝35.608，P＝0.000），而bcl-2、TS的表达降低（F＝30.048，P＝0.000；F＝22.874，P＝0.000）。结论抑制ANXA11表达能提高人胃癌细胞株SGC7901对5-Fu的敏感性，可能与抑制TS、bcl-2、上调bax的表达有关。ObjectiveTo investigate the effect of Annexin A11 （ANXA11） on 5-fluorouracil （5-Fu） resistance of human gastric cancer cell line SGC7901. MethodsThe expression of ANXA11 in human gastric cancer cell lines SCG7901 and BGC823 was detected. ANXA11-small interfering RNA （siRNA） and negative control sequences were transiently transfected into SGC7901 cells. cell counting kit-8 （CCK-8） assay was used to detect the sensitivity to 5-Fu with different dose （1, 10, 20, 40, 80 μg/ml） in each group. Apoptosis rate of each group was detected by flow cytometry. Quantitative polymerase chain reaction （qPCR） and Western blotting were used to detect the changes of B cell lymphoma/leukemia-2 associated X protein （bax）, B cell lymphoma/leukemia-2 （bcl-2） and TS. ResultsCompared with the BGC-823 cells, ANXA11 mRNA and protein expressions in SGC7901 cells were significantly higher （t=3.212, P=0.033; t=-10.768, P=0.000）. 50% inhibitory dose （IC50） and 20% inhibitory dose （IC20） of the transfection group （transfected with ANXA11-siRNA） was significantly lower than the negative control （transfected with NS-siRNA） and blank control group （F=47.500, P=0.000; F=45.523, P=0.000）. The apoptosis rate increased significantly in the transfection and the combined group （transfected with ANXA11-siRNA and combined with IC20 concentration of 5-Fu） compared with the negative control and the blank control group （F=117.525, P=0.000）, and the apoptosis rate of the combined group was significantly higher than that of the transfection group （P〈0.05）. The expression of ANXA11 protein decreased significantly in the transfection and the combined group （F=118.403, P=0.000）. Compared with the negative control group and the blank control group, the expression of bax in the transfection group and the combined group were up-regulated （F=35.608, P=0.000）, while those of bcl-2 and TS were significantly down-regulated （F=30.048, P=0.000; F=22.874, P=0.000）. ConclusionSuppres

关 键 词：膜联蛋白A11 胃癌 5-氟尿嘧啶 化疗耐药 RNA干扰技术 

分 类 号：R735.2[医药卫生—肿瘤]