骨髓间充质干细胞对胃癌细胞迁移和侵袭的影响及其机制  

Effect of bone marrow mesenchymal stem cells on migration and invasion of gastric cancer cells andmechanism

作　　者：张琳[1] 李良庆[1] 郑建涛[1] Zhang Lin;Li Liangqing;Zheng Jiantao(Departments of Gastrointestinal Surgery, the First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, Chin)

机构地区：[1]福建医科大学附属第一医院胃肠外科,福州350005

出　　处：《中华实验外科杂志》2018年第4期624-626,共3页Chinese Journal of Experimental Surgery

基　　金：福建省卫生与计划生育委员会青年科研课题（2016-1-53）

摘　　要：目的探讨骨髓间充质干细胞（BMSCs）对胃癌细胞SGC-7901、BGC-823迁移和侵袭的影响及机制。 方法采用Transwell小室将BMSCs与胃癌细胞共培养的方式，噻唑蓝（MTT）法检测细胞活力，划痕实验检测细胞迁移能力，Transwell实验检测细胞侵袭能力，Western blot检测细胞中基质金属蛋白酶（MMP）-2、MMP-9、磷脂酰肌醇3激酶（PI3K）/蛋白激酶B（Akt）信号通路相关蛋白的表达。 结果与BMSCs（－）组比较，BMSCs（＋）组胃癌细胞活力、迁移能力、侵袭能力均显著降低；在胃癌细胞SGC-7901中，MMP-2、MMP-9、PI3K以及磷酸化Akt（p-Akt）表达量分别为0.92±0.06、0.68±0.08、0.58±0.03、0.29±0.02，较对照组显著下调（P＝0.000、0.012、0.031、0.027）；在胃癌细胞BGC-823中，MMP-2、MMP-9、PI3K以及p-Akt表达量分别为0.50±0.05、0.26±0.04、0.31±0.02、0.13±0.03，较对照组均显著下调（P＝0.018、0.023、0.029、0.016）。 结论BMSCs可能通过阻断PI3K/Akt信号通路抑制胃癌细胞的迁移及侵袭。ObjectiveTo investigate the effect and mechanism of bone marrow mesenchymal stem cells （BMSCs） on the migration, invasion and invasion of gastric cancer cells （SGC-7901 and BGC-823）. MethodsTranswell chambers were used to culture BMSCs and gastric cancer cells. The cell vitality, migration ability, and invasion ability were measured by methyl thiazol tetrazolium （MTT） method, cratch test and Transwell assay, respectively. Western blotting was used to detect the expression of matrix metalloproteinase （MMP）-2 and MMP-9, phosphatidyl inositol 3 kinase （PI3K）/protein kinase B （Akt） signaling pathway. ResultsAs compared with BMSCs （-） group, cell vitality, and migration and invasion ability in BMSCs group （＋ ） were significantly reduced. The expression of MMP-2, MMP-9, PI3K, and p-Akt was 0.92±0.06, 0.68±0.08, 0.58±0.03 and 0.29±0.02 in SGC-7901 cells （P=0.000, 0.012, 0.031, 0.027）, and that was 0.50±0.05, 0.26±0.04, 0.31±0.02 and 0.13±0.03 in BGC-823 cells, which was significantly lower than in the control group （P=0.018, 0.023, 0.029, 0.016）. ConclusionBMSCs may inhibit the migration and invasion of gastric cancer cells by blocking the PI3K/Akt signaling pathway.

关 键 词：骨髓间充质干细胞 胃癌 迁移 侵袭 

分 类 号：R735.2[医药卫生—肿瘤]