M2型丙酮酸激酶和张力蛋白同源物基因在胃癌及癌旁组织的表达及临床意义  

Expression of M2 - pyruvate kinase, phosphatase and tensin homologue deleted on chromosome ten in gastric carcinoma tissues and non -cancerous adjacent gastric tissues

作　　者：黄哲 蔡朋株 林琳 徐飞鹏 许庆文 Huang Zhe;Cai Pengzhu;Lin Lin;Xu Feipeng;Xu Qingwen(Department of Gastrointestinal Surgery, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China)

机构地区：[1]广东医科大学附属医院胃肠外科,湛江524001

出　　处：《中华实验外科杂志》2018年第4期631-633,共3页Chinese Journal of Experimental Surgery

基　　金：广东省湛江市科技计划项目（2015801027）

摘　　要：目的检测胃癌及癌旁组织中M2型丙酮酸激酶（PKM2）和张力蛋白同源物基因（PTEN）的差异表达，探讨PKM2表达水平及PTEN表达水平与胃癌临床病理特征之间的关系。 方法应用免疫组织化学方法检测88例胃癌组织和癌旁组织中PKM2和PTEN表达，结合相关临床资料进行分析。 结果PKM2在胃癌组织的阳性表达率为71.59%（63/88），明显高于癌旁组织阳性表达率[30.68%（27/88）]，两者比较差异有统计学意义（t＝29.470，P＝0.000），PKM2表达水平与胃癌TNM分期、淋巴结转移呈明显相关（分别为t＝4.130，P＝0.040；t＝5.840，P＝0.020）；PTEN在胃癌组织的阳性表达率为39.77%（35/88），明显低于胃癌癌旁组织阳性表达率[92.05%（81/88）]，两者差异有统计学意义（t＝53.510，P＝0.000），PTEN表达水平与胃癌TNM分期、组织学分化程度、淋巴结转移呈明显相关（分别为t＝5.550，P＝0.020；t＝4.330，P＝0.040；t＝5.840，P＝0.020）。 结论PKM2和PTEN的表达水平可能与胃癌的发生、发展及淋巴结转移明显相关。ObjectiveTo investigate the expression and its clinical significance of M2-pyruvate kinase （PKM2） and phosphatase and tensin homologue deleted on chromosome ten （PTEN） in gastric carcinoma.MethodsThe expression levels of PKM2 and PTEN in 88 cases of gastric carcinoma tissues and adjacent gastric tissues were detected by immunohistochemistry.ResultsThe expression rate of PKM2 was 71.59% （63/88） in gastric carcinoma tissues, and 30.68% （27/88） in non-cancerous adjacent gastric tissues （t=29.470, P=0.000）. The expression of PKM2 was correlated to TNM stage and lymph node metastasis of gastric carcinoma （respectively, t=4.130, P=0.040; t=5.840, P=0.020）. The expression rate of PTEN was 39.77% （35/88） in gastric carcinoma tissues, and 92.05% （81/88） in non-cancerous adjacent gastric tissues （t=53.510, P=0.000）. The expression of PTEN was correlated to TNM stage, histology grade and lymph node metastasis of gastric carcinoma （t=5.550, P=0.020; t=4.330, P=0.040; t=5.840, P=0.020, respectively）.ConclusionThe expression of PKM2 and PTEN may be correlated to the carcinogenesis and progression of gastric carcinoma.

关 键 词：胃癌 M2型丙酮酸激酶 张力蛋白同源物基因 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤]