肿瘤坏死因子相关诱导凋亡配体联合顺铂对荷PC-9肺癌小鼠肿瘤生长的影响  

Tumor growth in bearing PC -9 mice on treatment of tumor necrosis factor - related apoptosis -inducing ligand combined with cisplatin and anti - tumor mechanism analysis

作　　者：张春敭[1] 齐宇 吴恺[1] 张岩[1] 赵松[1] Zhang Chnayang;Qi Yu;Wu Kai;Zhang Yan;Zhao Song(Department of Thoracic Surgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Chin)

机构地区：[1]郑州大学第一附属医院胸外一科,450052

出　　处：《中华实验外科杂志》2018年第4期699-701,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察肿瘤坏死因子相关诱导凋亡配体（TRAIL）联合顺铂对荷PC-9肺癌小鼠肿瘤生长的影响。 方法将60只荷PC-9肺癌肿瘤的裸鼠随机分为对照组、TRAIL组、顺铂组和联合治疗组，每组15只，TRAIL组经腹腔注射TRAIL 10 mg/kg，顺铂组经腹腔注射顺铂1.5 mg/kg，联合治疗组经腹腔分别注射TRAIL 10 mg/kg和顺铂1.5 mg/kg，对照组经腹腔注射等体积的生理盐水。所有小鼠每隔1 d给药1次，连续治疗30 d，测定每组小鼠肿瘤的生长情况和体重的变化。并采用原位缺口末端标记法（TUNEL）染色法分析4组小鼠肿瘤组织中细胞的凋亡水平。采用Western blot分析4组小鼠肿瘤组织细胞中p53、半胱氨酰天冬氨酸特异性蛋白酶（Caspase）-3等凋亡相关蛋白的表达水平。 结果与对照组比较，TRAIL组、顺铂组和联合治疗组小鼠肿瘤生长受到显著抑制（P＝0.030、0.026、0.009），而联合治疗组小鼠肿瘤生长速度明显缓于TRAIL组和顺铂组（P＝0.036、0.018）。与对照组比较，顺铂组和联合治疗组小鼠体重明显下降（P＝0.030、0.038），而TRAIL组小鼠体重变化差异无统计学意义（P＝0.070）。与对照组比较，TRAIL组、顺铂组和联合治疗组小鼠肿瘤组织中细胞凋亡比例明显增加（P＝0.023、0.034、0.027）。与顺铂组比较，TRAIL组和联合治疗组小鼠肿瘤组织细胞凋亡比例明显增加（P＝0.028、0.013）。Western blot结果显示与对照组比较，TRAIL组、顺铂组和联合治疗组p53和Caspase-3表达水平明显增加，而联合治疗组增加水平更加显著（P＝0.018）。 结论TRAIL联合顺铂能显著抑制荷PC-9小鼠肿瘤的生长，这一现象是通过促进肿瘤细胞凋亡相关基因表达，进而促进肿瘤细胞凋亡来实现的。ObjectiveTo explore Tumor growth in bearing PC-9 mice on treatment of tumor necrosis factor-related apoptosis-inducing ligand （TRAIL） combined with cisplatin and anti-tumor mechanism analysis. MethodsSixty nude mice bearing human lung adenocarcinoma PC-9 xenografted tumor were randomly divided into Control group, TRAIL group, cisplatin group and combination group. Mice in TRAIL group were treated with TRAIL （10 mg/kg） by intraperitoneal injection. Mice in cisplatin group were treated with cisplatin （1.5 mg/kg） by intraperitoneal injection. Mice in combination group were treated with TRAIL （10 mg/kg） and cisplatin （1.5 mg/kg） by intraperitoneal injection. Mice in control group were treated with phosphate buffer （PBS） by intraperitoneal injection. After 30 days of treatment, tumor growth, survival and body weight among four groups were analyzed. Apotosis of tumor cell in four groups were analyzed by terminal-deoxynucleotidyl transferase mediated nick end labeling （TUNEL） staining. Apotosis proteins p53 and Caspase-3 were analyzed by Western blotting. ResultsCompared with the control group, tumor growth in TRAIL group, cisplatin group and combination group were significantly inhibited （P=0.030, 0.026, 0.009）, while tumor growth rate in combination group was significantly slower than that of TRAIL group and cisplatin group （P=0.036, 0.018）. Compared with the control group, the body weight of the cisplatin group and the combined treatment group was significantly decreased （P=0.030, 0.038）, while there was no difference in body weight change （P=0.070） between TRAIL group and control group. Compared with control group, the proportion of apoptosis in TRAIL group, cisplatin group and combination group was significantly increased（P=0.023, 0.034, 0.027）. Compared with the cisplatin group, the apoptosis ratio of TRAIL group and combination group was significantly increased（P=0.028, 0.013）. Compared with control group, the expression of p53 and Caspase-3 in the TRAIL g

关 键 词：肿瘤坏死因子相关诱导凋亡配体 顺铂 凋亡 肿瘤生长 

分 类 号：R734.2[医药卫生—肿瘤]