血根碱对紫杉醇耐药卵巢癌A2780/taxol细胞生长及化疗敏感性影响的研究  

The Effect of Growth and Chemotherapy Sensitivity on Ovarian Cancer Cells A2780/taxol by Sanguinarine

作　　者：赵洪波 王妍妍[2] 冷天艳[2] 杨丽华[2] ZHAO Hongbo;WANG Yanyan;LENG Tianyan;et al(The Molecule Clinical Research Institute of Kunming Medical University, Kunming Yunnan 650500, China;The Second Affiliated Hospital of Kunming Medical University, Kunming Yunnan 650101 ,China)

机构地区：[1]昆明医科大学分子临床医学研究院,云南昆明650500 [2]昆明医科大学第二附属医院,云南昆明650101

出　　处：《实用妇产科杂志》2018年第4期268-272,共5页Journal of Practical Obstetrics and Gynecology

基　　金：云南省卫生和计划生育委员会医学学科带头人培养项目(编号:D-201633);国家自然科学基金地区项目(编号:81360336);云南省教育厅科学研究基金项目重点项目(编号:2015Z074);云南省科技厅-昆明医科大学应用基础研究联合专项资金面上项目[编号:2017FE467(-062)]

摘　　要：目的:探讨血根碱对紫杉醇耐药卵巢癌A2780/taxol细胞生长及化疗敏感性的影响和机制。方法:培养A2780/taxol细胞,分为对照组、血根碱组、紫杉醇组、血根碱+紫杉醇组,对照组加0.9%氯化钠液,血根碱组加2.0μmol/L血根碱,紫杉醇组加2μg/ml紫杉醇,血根碱+紫杉醇组同时加2.0μmol/L血根碱及2μg/ml紫杉醇。应用MTT法、流式细胞仪和Western blot法检测血根碱对A2780/taxol细胞增殖、凋亡、克隆形成及Bax、β-tubulinⅢ蛋白表达的影响。结果:与对照组相比,血根碱组、紫杉醇组及血根碱+紫杉醇组均能显著抑制A2780/taxol细胞增殖,血根碱+紫杉醇组抑制率最高(P<0.05);紫杉醇组、血根碱组及血根碱+紫杉醇组细胞克隆形成率[(35.67±7.37)%、(27.67±2.52)%和(8.87±3.38)%]明显降低,血根碱+紫杉醇组降低最为明显(P<0.05);紫杉醇组、血根碱组及血根碱+紫杉醇组细胞凋亡率[(18.01±7.13)%、(57.03±12.91)%和(81.68±11.23)%]明显增加,血根碱+紫杉醇组细胞凋亡率最高(P<0.05);紫杉醇组、血根碱组、血根碱+紫杉醇组Bax蛋白表达显著上调、β-tubulinⅢ蛋白表达显著降低,血根碱+紫杉醇组表达量变化最大(P<0.05)。结论:血根碱可下调β-tubulinⅢ表达,诱导肿瘤细胞凋亡,增强紫杉醇对耐药卵巢癌细胞的生长抑制作用,从而促进其对紫杉醇药物的敏感性。Objective：investigate the role and mechanism of sanguinarine to reverse paclitaxel drug resistance to paclitaxel resistant ovarian cancer ceil A2780/taxol by sanguinarine. Methods： Paclitaxel resistant ovarian cancer A2780/taxol cells were divided into control group, sanguinarine group, paclitaxel and paclitaxel combined sanguinarine group. The cells in control group were treated with NS ;The cells in sanguinarine group were treated with 2.0 μmol/L sanguinarine;The cells in paclitaxel group were treated with paclitaxel;the cells in the paclitaxel combined sanguinarine group were treated with 2.0 μmol/L paclitaxel and 2 μg/ml sanguinarine at the concentra- tion of at the same time. The proliferation, apoptosis and the expression of Bax, 15-tubulin m were determined by MTT method,the clone formation, Flow cytometry and Western blot. Results： Compared with control group,the proliferation of cells in the sanguinarine,paclitaxel and paclitaxel combined sanguinarine group were significantly inhibited（ P 〈 0.05 ）. The clone formation of cells were ~ （ 35.67 ± 7.37 ） %, （ 27.67 ± 2, 52 ） % and （ 8.87 ± 3.38） % ] in the sanguinarine, paclitaxel and paclitaxel combined sanguinarine group respectively,which were sig- nificantly decreased. The apoptosis rate were [ （18.01 ±7. 13） % , （57.03 ± 12.91 ） % and （81.68 ± 11.23） ] in the sanguinarine,paclitaxel and paclitaxel combined sanguinarine group respectively,which were increased sig- nificantly. The expression of Bax were up-regulated while the expression of 15-tubulin Ⅲ were down regulated. Theproliferation,clone formation,apoptosis rate,the expressions of Bax and β-tubulin β were significantly different a- mong them,which were most obvious in paclitaxel combined sanguinarine group （P 〈 0.05）. Conclusions： San- guinarine can down-regulate the expression of tubulin Ⅲ ,induce the apotosis and enhance the inhibition effect of A2780/taxol cells then promote paclitaxel drug sensitivity.

关 键 词：血根碱 紫杉醇 耐药 卵巢癌 化疗敏感性 

分 类 号：R737.31[医药卫生—肿瘤]