光炭轮菌菌丝体的化学成分  

Chemical composition of the mycelium of Daldinia eschscholtzii

作　　者：邱玲[1] 李莎[1] 李小华 杨岚 于思 罗禹 邓赟[1] QIU Ling;LI Sha;LI Xiaohua;YANG Lan;YU Si;LUO Yu;DENG Yun(Key Laboratory of Standardization of Chinese Herbal Medicine, Ministry of Education, Sichuan Provincial Key Laboratory of Systematic Research, Development and Utilization of Chinese Medicine Resources： Ministry of State Key Laboratory Breeding Base, Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China;School of Pharmacy, Southwest Medical University, Luzhou 646000, China)

机构地区：[1]成都中医药大学药学院中药材标准化教育部重点实验室、四川省中药资源系统研究与开发利用重点实验室-省部共建国家重点实验室培育基地,成都611137 [2]西南医科大学药学院,泸州646000

出　　处：《应用与环境生物学报》2018年第2期347-351,共5页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金面上项目(81373961);国家基础科学人才培养基金项目(J13100340);四川省青年科技创新研究团队专项计划(2014TD0007)资助~~

摘　　要：对光炭轮菌菌丝体乙醇提取物的乙酸乙酯萃取部位的化学成分进行研究. 采用色谱方法进行分离纯化，并利用质谱、核磁共振等波谱方法对化合物进行结构鉴定；采用MTT法对部分化合物进行肿瘤细胞的细胞毒活性测定. 共分离并鉴定了11个化合物：3β-羟基-5α,8α-过氧麦角甾-6,22-二烯（1）、9（11）-脱氢过氧麦角甾醇（2）、单花生酸甘油酯（3）、4,6,8（14）,22-四烯-3-麦角甾酮（4）、丁香脂素（5）、3,5,3′,5′-四甲氧基-4,4′-联苯二酚（6）、5-甲氧基香豆素（7）、5-羟基-2-甲基-4-色原酮（8）、（2R,4R）-3,4-二氢-5-甲氧基-2-甲基苯并二氢吡喃酮（9）、5-甲氧基-2-甲基二氢色原酮（10）、7β-caruilignan C（11）. 其中化合物4对肿瘤细胞H1299、H460、HGC-27、A549和MNK-45具有抑制作用，IC50值分别为25.2 ± 2.9、32.3 ± 4.2、29.2 ± 1.2、33.9 ± 3.7、18.0 ± 7.0 μmol/L. （表1 参34）To investigate the chemical composition of the fungus Daldinia eschscholtzii, the compounds were separated by silica gel, Sephadex LH-20, and preparative chromatography. Their structures were identified by spectral methods. The MTT method was applied to measure the cytotoxicity of representative components. Eleven compounds were isolated and identified as 3β-hydroxyl-6,22-dien-5α,8α-peroxynitrite （1）; ergosterol-9（11）-dehydroperoxide （2）; mangiferonic acid （3）; ergosta-4,6,8 （14）,22-tetraen-3-one （4）; （＋）-syringaresinol （5）; 3,5,3′,5′-tetramethoxy-4,4,-diphenol （6）; 5-methoxycoumarin （7）; 5-hydroxy-2-methyl-4H-chromen-4-one （8）; （2R,4R）-3,4-dihydro-5-methoxy-2-methyl-2H-1-benzopyran-4-ol （9）; 2,3-dihydro-5-methoxy-2-methylchromen-4-one （10）; and 7β-caruilignan C （11）, respectively. Compound 4 showed inhibitory activity against H1299, H460, HGC-27, A5491, and MNK-45, with the IC50 value of 25.2 ± 2.9, 32.3 ± 4.2, 29.2 ± 1.2, 33.9 ± 3.7, and 18.0 ± 7.0 μmol/L, respectively.

关 键 词：光炭轮菌 麦角甾醇 色原酮 联苯酚 细胞毒活性 

分 类 号：Q936[生物学—微生物学]