利用组织芯片分析DJ-1蛋白在非霍奇淋巴瘤组织中的定位表达及意义  

Analysis of the localization,expression and significance of DJ-1 protein in non-Hodgkin lymphoma tissue by tissue chip

作　　者：王文松 黄英[2] 李清叶 刘芳[1] 张杨[1] 肖国华[1] 谭晖[1] WANG Wensong, HUANG Ying, LI Qingye, LIU Fang, ZHANG Yang, XIAO Guohua, TAN Hui(Cancer Research Institute, School of Medicine, University of South China, Hengyang 421001, Hunan, Chin)

机构地区：[1]南华大学医学院肿瘤研究所,湖南衡阳421001 [2]常德职业技术学院 [3]湖南环境生物学院医学院

出　　处：《中南医学科学杂志》2018年第2期119-122,135,共5页Medical Science Journal of Central South China

基　　金：国家自然科学基金项目(81100375);湖南省教育厅课题(14C0140);湖南省研究生创新项目(课题编号:CX2017B553);衡阳市科技局项目(2015KJ20)

摘　　要：DJ-1是PARK7基因编码的一种蛋白质,其过表达与许多恶性肿瘤的发生、侵袭、转移及预后密切相关。然而,有关DJ-1蛋白在非霍奇金淋巴瘤(NHL)中的表达与NHL生物学特性的关系尚未见文献报导。本文检测了61例NHL组织中DJ-1蛋白在细胞核内外的表达情况,发现DJ-1蛋白在细胞浆内高表达的情况较细胞核内更为显著(93.4%、72.1%),且都显著高于非肿瘤组织(28.6%、14.3%)。此外,DJ-1蛋白的高表达率与NHL的恶性程度正相关,DJ-1蛋白在惰性、侵袭性和高侵袭性NHL中核高表达率为52.2%、82.9%和100%;浆高表达率为82.6%、100%和100%。结果提示DJ-1蛋白在NHL细胞内的定位可能是一个动态过程,而这与肿瘤的发生和发展密切相关;DJ-1与NHL的转移和侵袭等恶性进程密切相关,DJ-1是极具潜力的NHL相关的肿瘤生物学标记物。DJ-1/PARK7 is a gene with oncogenic potential,and its overexpression is strongly linked with the occurrence,invasion,metastasis and prognosis of numerous malignant tumors. However,the relationship between DJ-1 protein expression and the biological characteristics of non-Hodgkinlymphoma（NHL） was not previously reported in the literature.This research investigated the intracellular distribution of DJ-1 protein in NHL tissues obtained from 61 patients,finding that DJ-1 proteins located in the cytoplasm at a higher compression ratio than those in the nucleus（93.4%,72.1%,respectively）,and both were remarkably higher than those in non-tumor tissues（28.6%,14.3%,respectively）. In addition,tissue expression of DJ-1 protein is positive to the malignancy of NHL： for indolent,aggressive and highly aggressive NHL,DJ-1 protein expressing rates in the cytoplasm were 52.2%,82.9% and 100%,and in the nucleus were 82.6%、100% 和100%,respectively.The results indicate that the localization of DJ-1 protein in NHL cells may be a dynamic process,which is closely associated with development and progression of lymphoma; DJ-1 is closely related to malignant processes such as metastasis and invasion of NHL; DJ-1 is a promising NHL-related oncology biomarker.

关 键 词：非霍奇金淋巴瘤 组织芯片 DJ-1蛋白表达 

分 类 号：R361.2[医药卫生—病理学]