鹿源高效纤维素降解菌的筛选和鉴定  

Screening and identification of efficient cellulose degrading bacteria from sika deer

作　　者：韦海婷 李光玉[1] 金春爱[1] 刘晓颖[1] WEI Haiting;LI Guangyu;LIU Xiaoying(Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China)

机构地区：[1]中国农业科学院特产研究所

出　　处：《黑龙江畜牧兽医》2018年第7期162-166,251,252,共7页Heilongjiang Animal Science And veterinary Medicine

基　　金：中央级公益性科研院所基本科研业务费专项(201720)

摘　　要：为了探究梅花鹿瘤胃内的纤维素降解菌,试验采取梅花鹿瘤胃液进行富集培养,利用羧甲基纤维素钠（CMC-Na）培养基分离菌株,通过刚果红染色产透明圈进行初筛,结合其纤维素酶测定进行复筛,最终筛选出一株分解纤维素能力最强的菌株。结果表明：筛选菌株为枯草芽孢杆菌,该菌株的透明圈直径（D）与菌落直径（d）的比值（D/d）为4.83;在40℃、pH值为6.0-7.0范围内环境培养48 h纤维素酶（CMCA）酶活和滤纸酶（FPA）酶活达到最大值,分别为18.378 U/m L、6.090 U/mL;CMC-Na的接种量在1%-2%范围内CMCA酶活和FPA酶活较高;在以CMC-Na为碳源时,CMCA和FPA的酶活比滤纸、麸皮、豆粕作为碳源时的值大。说明该菌株具有分解纤维素的能力。The aim of the present study was to identify the cellulose degrading bacteria in the rumen of sika deer. CMC-Na medium was used to isolate bacterial strains from the collected rumen fluid of sika deer. Congo red staining method was used for preliminary screen of the strains,then filter paper enzyme activity and CMC enzyme activity of cellulase were further determined. The results showed that the bacterial strain Bs was isolated and identified as Bacillus subtilis. The ratio（D/d） of transparent zone diameter（D） to colony diameter（d） was 4. 83. The production of the cellulase could reach the peak at 48 h. When fermentation temperature was 40 ℃ and pH was 6. 0-7. 0,the highest enzyme activity could be observed. They were 18. 378 U/mL and 6. 090 U/mL,respectively. The activity of cellulase and filter paper enzyme was higher in the range of 1% and 2% of CMC-Na. When CMC-Na was used as carbon source,the enzyme activity of CMCA and FPA was higher than that of filter paper,wheat bran and soybean meal as carbon source. The results indicated that the strain had the ability of degrading cellulose.

关 键 词：梅花鹿 瘤胃 纤维素降解菌 纤维素酶(CMCA) 分离 筛选 枯草芽孢杆菌 

分 类 号：Q935[生物学—微生物学]