1株野生鼠诺如病毒的全基因组序列测定及分析  

Complete genomic sequence analysis of a GV Murine Norovirus in wild mice

作　　者：刘蒙蒙 李利利[2] 林琳 王笑峰[1] 段招军[2] Liu Mengmeng;Li Lili;Lin Lin;Wang Xiaofeng;Duan Zhaojun(Department of Medical Microbiology, Qingdao University Medical College, Qingdao 266021, China;Department of Diarrhea, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China;Shandong Provincial Center for Disease Control and Prevention, Academy of Preventive Medicine of Shandong University, Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Jinan 250014 Chin)

机构地区：[1]青岛大学基础医学院病原生物学系,山东266021 [2]中国疾病预防控制中心病毒病预防控制所腹泻室,北京100052 [3]山东省疾病预防控制中心山东大学预防医学研究院山东省传染病预防控制重点实验室,济南250014

出　　处：《中华实验和临床病毒学杂志》2018年第2期176-180,共5页Chinese Journal of Experimental and Clinical Virology

基　　金：国家自然科学基金（81290345）;国家重点研究计划生物安全关键技术研发重点专项（2016YFC1201904）

摘　　要：目的为确定1株通过高通量测序获得的GV型野生鼠诺如病毒（MNV）变异株的基因组序列特征。方法将在山东地区采集的210只鼠经解剖后收集的肠道内容物和肝脏进行核酸提取，质检合格后送于华大基因进行高通量测序，获得一株GV型野生鼠诺如病毒。设计特异性巢式引物利用RT-PCR方法扩增该病毒全基因组。利用DNAMAN，MEGA5．0软件进行序列比对及系统进化分析。结果获得的GV型野生MNV变异株全基因组长度7382bp，与从数据库中选取的30株参考毒株全基因组序列相似度为76．8％～78．1％，其中VP1中的高变区P2编码的氨基酸相似度仅为60．9％～78．1％，表明该株MNV具有较高的变异率。结论本研究分离出了一株野生鼠诺如病毒，丰富了国内MNV的基因库，其变异特征为了解国内MNV的分子遗传特征及进化来源提供了参考依据。Objective To identify the molecular structure and genome homology characteristics of a GV murine norovirus （MNV） from wild miee. Methods Nucleic acids were extracted from collected mouse intestinal specimens and subjected to high-throughput sequeneing. The whole genome sequence was obtained by RT-PCR and RACE method . Results The MNV genome was 7,832 bp in length, with 76.8% -78. 1% nucleotide identity to other seleeted murine norovirus strains in GenBank. The P2 region, the hypervariable area in viral protein 1 gene shows highest heterology, and the identity was among 60.9% -78.1% , lower than other genes regions. The result demonstrated the higher variability of this newly isolated WNV strain. Conclusions The wild-type MNV isolated in this study is first reported in China, which enriehed the domestic MNV gene pool and provided a referenee for understanding the molecular genetic eharacteristics and evolutionary origin of MNV in China.

关 键 词：鼠诺如病毒 全基因组 系统进化分析 

分 类 号：R373[医药卫生—病原生物学]