酵母菌0939-5对葡萄灰霉病菌抑制作用研究  

Inhibitory effect of yeast 0939-5 on Botrytis cinerea

作　　者：张迪 王晓东 Zhang Di;Wang Xiaodong(Key Laboratory of Prevention and Control for Oasis Crop Disease, Shihezi University, Shihezi Xinjiang 832000, Chin)

机构地区：[1]石河子大学绿洲农作物病害防控重点实验室,新疆石河子832000

出　　处：《中国植保导刊》2018年第4期5-10,共6页China Plant Protection

基　　金：公益性行业(农业)科研专项(201303025)

摘　　要：采用双皿对扣法和平板隔离对峙法研究了酵母菌0939-5对葡萄灰霉菌菌丝生长和孢子萌发的抑制作用,并根据其产生的挥发性物质,以活性炭处理检验其对葡萄灰霉病菌丝和分生孢子的抑制作用。结果表明,酵母菌0939-5产生的挥发性物质对葡萄灰霉病菌的生长(抑制率为71.59%)及其分生孢子(抑制率为99.33%)的萌发具有显著的抑制作用;添加活性炭吸附后,葡萄灰霉病菌菌丝的生长及分生孢子的萌发恢复了活性,显著降低了抑制作用。同时,酵母菌0939-5产生的挥发性物质对7种病原真菌也具有明显的抑制作用,尤其对棉花立枯丝核菌病菌(Rhizoctonia solani Kühn)、番茄早疫病菌(Alternaria solani)稻瘟病菌(Magnaporthe oryzae)、棉花黄萎病菌(Verticillium dahlia)的抑菌效果较好,其抑菌率达90.93%~94.00%。Inhibitory effect of yeast 0939-5 on mycelial growth and spore germination of Botrytis cinerea were studied by two-sealed-base-plates method and plate isolation-confrontation method, which tested the function of volatile organic com- pounds （VOCs） produced by the yeast controlled with activated carbon. The results showed that the VOCs had a significant inhibitory effect on growth of B. cinerea （with inhibition rate of 71.59%） and conidial germination （with inhibition rate of 99.33%）. With adsorption of activated carbon, growth and conidial germination of B. cinerea resumed. And the VOCs also had a significant inhibitory effect on the growth of seven kinds of pathogenic fungi. Especially, it had good anti-fungal activity against Rhizoctonia solani Kuhn, A lternaria solani, Magnaporthe oryzae and Verticillium dahlia, with the anti-fun- gal rates of 90.93%-94.00%.

关 键 词：酵母菌0939-5 葡萄灰霉病菌 挥发性物质 抑制作用 

分 类 号：S436.631[农业科学—农业昆虫与害虫防治]