阿卡波糖调节2型糖尿病大鼠肠道SGLT1表达的影响  

Influence of acarbose on expression of intestinal SGLT1 in rats with type 2 diabetes mellitus

作　　者：马丽娜[1] 毕会民[1] 高凌[1] MA Li-na;BI Hui-min;GAO Ling(Department of Endocrinology, Renmin Hospital of Wuhan University, Wuhan Hubei, 430060, Chin)

机构地区：[1]武汉大学人民医院内分泌科,湖北武汉430060

出　　处：《职业与健康》2018年第6期753-756,共4页Occupation and Health

基　　金：国家自然科学基金资助项目(81571376)

摘　　要：目的探讨2型糖尿病大鼠模型的建模方法,对比阿卡波糖(拜唐苹)与格列美脲调节2型糖尿病大鼠的血糖水平,探讨阿卡波糖对2型糖尿病大鼠肠道钠-葡萄糖协同转运蛋白1(SGLT1)表达的影响。方法将40只SD雄性大鼠随机分为5组:正常对照组、正常对照+拜唐苹干预组、2型糖尿病模型对照组、糖尿病模型+拜唐苹干预组和糖尿病模型+格列美脲干预组,每组8只。糖尿病组的大鼠均给予高脂乳剂灌胃和高糖饮水,连续灌胃4周后,模型组大鼠一次性腹腔注射小剂量链脲佐菌素(STZ)以建立2型糖尿病大鼠模型。建模成功后,连续药物干预4周,观察大鼠血糖水平变化,运用反转录定量PCR(q RT-PCR)和免疫组化技术分别检测大鼠肠道组织SGLT1基因和蛋白的表达水平。结果与正常对照组比较,2型糖尿病模型对照组大鼠血糖明显升高78%,并显著上调肠道组织中SGLT1基因和蛋白的表达(P<0.05)。给药4周后,与模型对照组比较,拜唐苹干预组和格列美脲干预组明显降低糖尿病大鼠的血糖,拜唐苹干预组降低46%,格列美脲干预组降低24%,拜唐苹干预组和格列美脲干预组能够下调2型糖尿病大鼠肠道组织中SGLT1基因和蛋白的表达(P<0.05),在相同血糖水平下,拜唐苹干预组效果更显著。结论对SD大鼠给予高脂乳剂灌胃联合一次性小剂量STZ注射可以成功诱导2型糖尿病模型。拜唐苹和格列美脲对2型糖尿病大鼠有降低血糖的治疗作用,并且对糖尿病大鼠肠道SGLT1受体的表达有一定的影响。在相同血糖水平下,比较格列美脲和拜唐苹的干预效果显示,拜唐苹对2型糖尿病大鼠肠道SGLT1表达的影响更显著。[Objective] To investigate the modeling method of rats with type 2 diabetes mellitus,compare the effect between acarbose（glucobay） and glimepiride on blood glucose level of rats with type 2 diabetes mellitus,and explore the influence of acarbose on expression of intestinal sodium-glucose co transporter 1（SGLT1） in rats with type 2 diabetes mellitus.[Methods]40 SD male rats were randomly divided into five groups： normal control group,normal control ＋ acarbose intervention group,type 2 diabetic model group,diabetic model ＋ acarbose intervention group,diabetic model ＋ glimepiride intervention group,8 rats in each group. The rats in the diabetic group were treated with the high fat emulsion gavage and high glucose drinking water. After 4 weeks of continuous intragastric administration,the rats in the model group were injected intraperitoneally with small dose of streptozotocin（STZ）to establish the model of type 2 diabetes mellitus. After successful modeling,the blood glucose levels of rats were observed for 4 weeks after continuous drug intervention. The expression levels of SGLT1 gene and protein in the intestinal tissues of rats were detected by q RT-PCR and immunohistochemistry techniques.[Results]Compared with the normal control group,the blood glucose of type 2 diabetes model group was increased by 78%,and the expression of SGLT1 gene and protein in intestinal tissue significantly increased（P0.05）. After 4 weeks of treatment,compared with model group,the blood glucose levels of diabetic rats in acarbose intervention group and glimepiride intervention group significantly reduced,which decreased by 46% and 24%respectively,and expressions of SGLT1 gene and protein in intestinal tissue of rats with type 2 diabetes mellitus in acarbose intervention group and glimepiride intervention group decreased（P 0.05）. At the same level of blood glucose,the acarbose intervention group showed more significant effect.[Conclusion]The type 2 diabetes model is successfully induced in SD rats by intragastri

关 键 词：阿卡波糖 2型糖尿病大鼠 SGLT1 格列美脲 空肠 

分 类 号：R-332[医药卫生]