糖氧剥夺/再灌注抑制L2.3神经干细胞N-Myc-DLL3-Notch信号系统  

Glucose Deprivation/Reperfusion Inhibits N-Myc-DLL3-Notch Signaling System of L2.3 Neural Stemcells

作　　者：吴兰 江晓琴 周文琴 倪娟 童煜 Wu Lan;Jiang Xiaoqin;Zhou Wenqin;et al(West China second Hospital of Sichuan University Chengdu , Sichuan 610041, China)

机构地区：[1]四川大学华西第二医院麻醉科西部妇幼医学研究院出生缺陷与相关妇儿疾病教育部重点实验室,四川成都610041

出　　处：《四川医学》2018年第4期365-367,共3页Sichuan Medical Journal

基　　金：四川省科技厅应用基础研究项目资助(编号:2015JY0064);四川省科技厅支撑计划项目(编号:2015FZ0075)

摘　　要：目的建立L2.3神经干细胞糖氧剥夺/再灌注损伤模型,探讨糖氧剥夺/再灌注是否可以抑制L2.3神经干细胞N-Myc-DLL3-Notch信号系统。方法体外培养的L2.3神经干细胞经历糖氧剥夺(OGD)6h,再灌注(R)16h,建立OGD/R模型。分别在OGD前、OGD6h、OGD6h再灌注16h,用western blot方法检测Notch1、NICD、DLL3和N-Myc蛋白表达情况。结果与糖氧剥夺/再灌注前比,糖氧剥夺6h,再灌注16h可下调Notch1、NICD、DLL3和N-Myc蛋白表达。结论糖氧剥夺/再灌注损伤可抑制L2.Objective To establish L2.3 neural stem cells model of glucose and oxygen deprivation/reperfusion injury,and to investigate whether glucose deprivation/reperfusion can inhibit N-Myc-DLL3-Notch signaling system of L2.3 neural stem cells.Methods L2. 3 neural stem cells in vitro cultured were given oxygen glucose deprivation（ OGD） 6h and reperfusion（ R） 16 h,and then a OGD/R model was established.The expression of Notch1、NICD、DLL3 and N-Myc protein was detected by western blot method at before OGD,OGD6h、16h after reperfusion,respectively.Results Compared with before oxygen and glucose deprivation/reperfusion,the expression of Notch1、NICD、DLL3 and N-Myc protein decreased after 6h of glucose and oxygen and glucose deprivation and 16h of reperfusion. Conclusion Oxygen and glucose deprivation/reperfusion injury can inhibit N-MycDLL3-Notch signaling system of L2.3 neural stem cells.

关 键 词：糖氧剥夺/再灌注 L2.3细胞 NOTCH1 DLL3 N-MYC 

分 类 号：Q735[生物学—分子生物学]