前列腺癌组织Livin表达与PTEN相关性研究  

Expressions of Livin in prostate carcinoma tissue and its correlation with PTEN expression

作　　者：周留正[1] 陈兵海[1] 孙浩[1] 潘鹏[1] 唐爱国[1] 贾俊海[2] ZHOU Liu-zheng;CHEN Bing hai;SUN Hao;PAN Peng;TANG Ai-guo;JIA Jun-hai(Department of Urology ,Affiliated Hospital of Jiangsu University ,Zhenjiang 212001 ,P. R. China;Medical College of Jiangsu University ,Zhenjiang 212013 P. R. China)

机构地区：[1]江苏大学附属医院泌尿外科,江苏镇江212001 [2]江苏大学医学院,江苏镇江212013

出　　处：《中华肿瘤防治杂志》2017年第23期1651-1655,共5页Chinese Journal of Cancer Prevention and Treatment

基　　金：江苏大学临床医学专项基金(JDLCZX006)

摘　　要：目的凋亡抑制蛋白(inhibitor of apoptosis protein,IAP)家族新成员Livin与前列腺癌(prostate carcinoma,Pca)关系密切,本研究探讨Livin在前列腺癌组织中的表达及其与抑癌基因PTEN的相关性。方法选取江苏大学附属医院2015-03-12—2016-10-15前列腺癌组织31例及前列腺增生(benign prostatic hyperplasia,BPH)组织18例,蛋白质印迹法和免疫组化SP法检测组织中Livin和PTEN的表达,分析前列腺癌组织中Livin和PTEN表达与临床病理参数之间的关系及Livin表达与PTEN表达的相关性。结果蛋白质印迹结果显示,前列腺癌组织Livin蛋白表达量为2.16±0.83,高于前列腺增生组织1.43±0.74,t=3.085,P=0.003;前列腺癌组织PTEN蛋白表达量为1.33±0.88,低于前列腺增生组织2.13±1.51,t=-2.351,P=0.023;免疫组化SP法显示,前列腺癌组织中Livin阳性表达与临床分期和转移显著相关,均P<0.05;但Livin与年龄、前列腺体积、病理分级和前列腺特异抗原(prostate specific antigen,PSA)无明显相关,均P>0.05;前列腺癌组织中PTEN蛋白的阳性表达与临床分期和转移显著相关,均P<0.05;但PTEN蛋白阳性表达与年龄、前列腺体积、病理分级和PSA无明显相关,均P>0.05;在前列腺癌组织中PTEN低表达,Livin高表达,两者表达呈显著负关联,OR=0.039 2,P=0.000 3。结论前列腺癌组织中Livin高表达,Livin表达与临床分期和转移显著相关;前列腺癌组织中PTEN低表达,PTEN表达与临床分期和转移显著相关;前列腺癌组织中Livin表达与PTEN表达呈显著负关联,Livin和PTEN与前列腺癌的发生、进展和转移密切相关。OBJECTIVE To study expressions of Livin,a novel member of inhibitor of apoptosis protein family,in prostate carcinoma tissue and to investigate its correlation with PTEN expression.METHODS Livin and PTEN were determined by Western blot in 31 cases of prostate carcinoma tissue and 18 cases of benign prostatic hyperplasia tissue,which were collected in Affiliated Hospital of Jiangsu University between 2015-3-12 and 2016-10-15.Immunohistochemistry（SP method）was also applied to detect the expression of Livin and PTEN protein in prostate carcinoma and to study the correlation between the Livin and PTEN.The association between the expression levels of Livin,PTEN and the clinicopathological characteristics were studied.RESULTS The expressions of Livin protein were significantly higher in prostate carcinoma than those in the benign prostatic hyperplasia tissue（2.16±0.83 vs 1.43±0.74,t=3.085,P=0.003）.The expressions of PTEN protein were significantly lower in prostate carcinoma than those in the benign prostatic hyperplasia tissue（1.33±0.88 vs 2.13±1.51,t=-2.351,P=0.023）.The level of Livin and PTEN were close related to clinical stage and metastasis;but have no correlation with gender,tumor location,differentiation degree and PSA.The Livin protein was highly expressed in prostate carcinoma tissue.The PTEN protein was lower expressed in prostate carcinoma tissue.The expression of Livin had negative correlation with the expression of PTEN（OR=0.0392,P=0.0003）.CONCLUSIONS The over expression of Livin and the low expression of PTEN in prostate carcinoma tissue are closed related to clinical stage and metastasis.The low expression of PTEN in prostate carcinoma tissue had negative correlation with the expression of Livin.The negative correlation between Livin and PTEN indicates that they are related to each other in carcinogenesis.The Livin and PTEN may play important roles in occurrence and development of prostate cancer.

关 键 词：前列腺癌 LIVIN PTEN 蛋白质印迹 免疫组织化学 

分 类 号：R737.25[医药卫生—肿瘤]