G蛋白偶联受体4对人乳头瘤病毒感染宫颈癌组织新生血管建立的影响和诊断价值  

Effects and diagnostic value of G protein-coupled receptor 4 on the establishment of neovascularization of human papillomavirus infection in cervical cancer tissues

作　　者：李付连 索玉平[2] 王谋香 王玉秀 李丽花 王鑫 Li Fulian;Suo Yuping;Wang Mouxiang;Wang Yuxiu;Li Lihua;Wang Xin(Department of Obstetrics and Gynecology, Lan County Maternal and Child Health and Family Planning Service Center, Lvliang 033500, Chin)

机构地区：[1]山西省吕梁市岚县妇幼保健和计划生育服务中心,033500 [2]山西省人民医院妇产科 [3]山西省吕梁市岚县人民医院妇产科 [4]山西省吕梁市岚县妇幼计生中心妇产科

出　　处：《中国综合临床》2018年第3期214-217,共4页Clinical Medicine of China

基　　金：山西省自然科学基金面上项目（201601D011089）;山西省自然科学基金青年基金项目（2014011039-6）;山西省自然科学基金青年基金项目（2013011045-5）;山西省自然科学基金青年基金项目（2011011037-1）;国家自然科学基金面上项目（30371483）;国家自然科学基金面上项目（30860304）

摘　　要：目的探究G蛋白偶联受体4（G-protein coupled receptor 4，LGR4）对人乳头瘤病毒（human papilloma virus，HPV）感染宫颈癌组织新生血管建立的影响和诊断价值。方法选取自2016年3月至2017年12月间来我院接受检查的HPV感染的宫颈癌患者、宫颈癌前病变者和宫颈健康者各30例作为研究对象，经研究对象本人同意后进行标本采集。免疫组化法观察三种组织标本的LGR4的阳性率，筛检试验观察LGR4在宫颈癌的分化、分期和分型中的诊断价值，观察LGR4阳性组织中相关促血管生成因子的表达。结果健康组织、癌前病变组织和宫颈癌组织中LGR4表达差异有统计学意义（χ2＝24.104，P＝0.000），且宫颈癌组织内LGR4的表达阳性率80.0%（24/30）显著高于健康组16.7%（5/30）和癌前病变组织50.0%（15/30），差异均有统计学意义（P均〈0.05）。LGR4的灵敏度80.00%，特异度83.33%，阳性预测值82.76%，阴性预测值80.65%，诊断准确率81.67%和Kappa值为0.63，一致性良好。不同年龄、不同分化程度和不同临床分期LGR4阳性率相比，差异均有统计学意义（P均〈0.05）。实验室结果显示，在宫颈癌前病变组织和宫颈癌LGR4阳性组织中超敏C反应蛋白、降钙素原、血管内皮因子和生长因子β均显著高于LGR4阴性组织，差异均有统计学意义（t值分别为9.247、2.925、39.669、15.348，P均〈0.05）。结论LGR4能够促进宫颈癌组织内新生血管的建立，对宫颈癌和宫颈癌前病变具有一定的诊断价值。ObjectiveTo explore the effect and diagnostic value of G protein coupled receptor 4 （LGR4） on the establishment of neovascularization of HPV infected cervical cancer tissue.MethodsFrom March 2016 to December 2017, thirty cases of HPV infected cervical cancer, cervical precancerous lesion and cervical health in Lan County Maternal and Child Health and Family Planning Service Center were selected as subjects.After the consent of the subjects, specimens were collected.Immunohistochemical method was used to observe the positive rate of LGR4 in three kinds of tissue specimens.The diagnostic value of LGR4 in differentiation, staging and typing of cervical cancer was observed by screening test, and the expression of related pro-angiogenic factors in LGR4-positive tissues was observed.ResultsThe difference in the expression of LGR4 in healthy tissue, precancerous lesions and cervical cancer tissues was statistically significant（χ2=24.104, P=0.000）. The positive rate of LGR4 expression in cervical cancer tissue was 80% （24/30）, which was significantly higher than that in healthy group 16.7% （5/30） and precancerous lesion tissue 50% （15/30）, the differences were statistically significant （P〈0.05）. The sensitivity of LGR4 was 80.00%, the specificity was 83.33%, the positive predictive value was 82.76%, the negative predictive value was 80.65%, the diagnostic accuracy was 81.67% and the Kappa value was 0.63, which has good consistency.The differences of LGR4 positive rate in different age, different degree of differentiation and different clinical stages were statistically significant （P〈0.05）. Laboratory results showed that CRP, PCT, VEGF and TGF-β in LGR4-positive tissues of cervical precancerous lesions and cervical cancer were significantly higher than those in LGR4-negative tissues, the differences were statistically significant （t=9.247, 2.925, 39.669, 15.348, P〈0.05）.ConclusionLGR4 can promote the establishment of neovascularization in cervical cancer and has certain diagnostic

关 键 词：G蛋白偶联受体4 HPV感染 宫颈癌 血管建立 诊断价值 

分 类 号：R737.33[医药卫生—肿瘤]