Protective effects of imperatorin against cerebral ischemia/reperfusion-induced oxidative stress through Nrf2 signaling pathway in rats  

作　　者：Wei HE Wei-wei CHEN Xian-hua HUANG Yu-mei ZHOU Fang LIAO 

机构地区：[1]Department of Pharmacology,Gannan Medical University,Key Laboratory of Cerebrovascular Pharmacology of Jiangxi Province

出　　处：《中国药理学与毒理学杂志》2017年第10期988-988,共1页Chinese Journal of Pharmacology and Toxicology

基　　金：supported by National Natural Science Foundation of China(81060269 and81360492);Natural Science Foundation of Jiangxi Province of China(20122BAB205036)

摘　　要：OBJECTIVE To investigates the effects of imperatorin on the oxidative stress in the cerebral cortex and hippocampus after focal cerebral ischemia/reperfusion injury.METHODS Transient focal cerebral ischemia/reperfusion model in male Sprague-Dawley rats was induced by 2 h middle cerebral artery occlusion followed by 24 h reperfusion.Imperatorin(1.25 and 2.5 mg·kg-1)or vehicle were administered intraperitoneally at 1,5 and 9 h after the onset of ischemia.At 24 h after reperfusion,the biomarkers of oxidative stress such as the levels of reactive oxygen species(ROS),lipid peroxidation products malondialdehyde(MDA),nitric oxide(NO)and total antioxidant capacity(T-AOC),the activities of inducible nitric oxide synthase(iN OS),superoxide dismutase(SOD)and catalase(CAT)in the cerebral cortex and hippocampus were observed.We also assessed the nuclear factor erythroid 2-related factor 2(Nrf2),heme oxygenase-1(HO-1),and the NAD(P)H-quinone oxidoreductase 1(NQO-1)protein expression by Western blot.RESULTS As compared to vehicle-treated animals,imperatorin treatment significantly reduced the ROS,MDA,NO levels and i NOS activity,increased T-AOC and the activities of SOD and CAT.Furthermore,imperatorin treatment also significantly induced the nuclear translocation of Nrf2,enhanced the protein expression of HO-1 and NQO-1 in the cerebral cortex and hippocampus.CONCLUSION Our findings indicate that imperatorin can protect the brain against the excessive oxidative stress induced by cerebral ischemia/reperfusion through activation of Nrf2 signaling pathway.OBJECTIVE To investigates the effects of imperatorin on the oxidative stress in the cerebral cortex and hippocampus after focal cerebral ischemia/reperfusion injury.METHODS Transient focal cerebral ischemia/reperfusion model in male Sprague-Dawley rats was induced by 2 h middle cerebral artery occlusion followed by 24 h reperfusion.Imperatorin（1.25 and 2.5 mg·kg-1）or vehicle were administered intraperitoneally at 1,5 and 9 h after the onset of ischemia.At 24 h after reperfusion,the biomarkers of oxidative stress such as the levels of reactive oxygen species（ROS）,lipid peroxidation products malondialdehyde（MDA）,nitric oxide（NO）and total antioxidant capacity（T-AOC）,the activities of inducible nitric oxide synthase（iN OS）,superoxide dismutase（SOD）and catalase（CAT）in the cerebral cortex and hippocampus were observed.We also assessed the nuclear factor erythroid 2-related factor 2（Nrf2）,heme oxygenase-1（HO-1）,and the NAD（P）H-quinone oxidoreductase 1（NQO-1）protein expression by Western blot.RESULTS As compared to vehicle-treated animals,imperatorin treatment significantly reduced the ROS,MDA,NO levels and i NOS activity,increased T-AOC and the activities of SOD and CAT.Furthermore,imperatorin treatment also significantly induced the nuclear translocation of Nrf2,enhanced the protein expression of HO-1 and NQO-1 in the cerebral cortex and hippocampus.CONCLUSION Our findings indicate that imperatorin can protect the brain against the excessive oxidative stress induced by cerebral ischemia/reperfusion through activation of Nrf2 signaling pathway.

关 键 词：IMPERATORIN cerebral ischemia/reperfusion reactive oxygen species nuclear factor erythroid 2-related factor 2 

分 类 号：R285.5[医药卫生—中药学]