鞘内注射帕瑞昔布钠在大鼠骨癌痛模型中的镇痛作用  

Effect of intrathecal parecoxib sodium in bone tumor pain of rats

作　　者：张乙[1] 隋成[1] 崔金花[1] Zhang Yi;Sui Cheng;Cui Jinhua.(Department of Anesthesiology, Dalian University Affiliated Xinhua Hospital, Dalian 116021, China)

机构地区：[1]大连大学附属新华医院麻醉科,116021

出　　处：《中国医师进修杂志》2018年第2期101-105,共5页Chinese Journal of Postgraduates of Medicine

摘　　要：目的研究鞘内注射帕瑞昔布钠在大鼠骨癌痛模型中的镇痛作用。方法选取40只6周雌性SD大鼠，在七氟烷麻醉下于胫骨注入MRMT-1（1 × 104/L）大鼠乳腺癌细胞建立骨癌痛模型，运用放射线和HE染色法来判断骨癌形成与否。注入MRMT-1后第3天，将PE-10导管置入蛛网膜下隙，用于鞘内注射0.9%氯化钠或帕瑞昔布钠。第10天，将大鼠按随机数字表法分为五组，对照组和帕瑞昔布钠0.1、0.3、1.0和3.0 g/L组，每组8只。通过von Frey纤维细丝测定大鼠机械缩爪阈值，观察鞘内注射帕瑞昔布钠的镇痛效果。结果放射线、病理学以及行为学结果共同表明注入MRMT-1肿瘤细胞后100%成功建立骨癌痛模型。对照组和帕瑞昔布钠0.1、0.3、1.0和3.0 g/L组的最大镇痛效应百分比分别为（13.89 ± 4.17）%、（7.54 ± 3.91）%、（57.47 ± 11.47）%、（85.72 ± 9.42）%和（100.00 ± 0.00）%，与对照组相比，鞘内注射帕瑞昔布钠能剂量依赖性地增加大鼠机械缩爪阈值（P〈0.05）。结论鞘内注射帕瑞昔布钠有效抑制大鼠骨癌痛。ObjectiveTo investigate the effect of intrathecal parecoxib sodium in bone tumor pain of rats.MethodsBone tumor pain was induced by injection of MRMT-1 tumor cells （1 × 104/L） into the tibia of female SD rats under sevoflurane anesthesia. The development of bone tumor was monitored by radiological study, and histological sections stained with hematoxylin and eosin. At 3 d after MRMT-1 tumor cell injection, a PE-10 catheter was inserted into the intrathecal space for drug administration. At 10 d after MRMT-1 tumor cell injection, rats were randomly divided into 5 equal groups, control group, parecoxib sodium 0.1, 0.3, 1.0 and 3.0 g/L group. For pain assessment, a withdrawal threshold was measured using von Frey filament being applied to the tumor cell inoculation site. The effects of intrathecal saline or parecoxib sodium were investigated.ResultsIntra-tibial injection of MRMT-1 tumor cells produced a bone tumor in radiologic and pathologic findings. Also, the paw withdrawal threshold was significantly decreased （mechanical allodynia）. Percentage of the maximal possible effect （% MPE） of control group and parecoxib sodium 0.1, 0.3, 1.0 and 3.0 g/L group was （13.89 ± 4.17）%, （7.54 ± 3.91）%, （57.47 ± 11.47）%, （85.72 ± 9.42）% and （100.00 ± 0.00）%, compared with control group, intrathecal parecoxib sodium dose-dependently increased the withdrawal threshold （P 〈 0.05）.ConclusionsIntrathecal parecoxib sodium reduces bone tumor-related pain behavior.

关 键 词：骨癌痛 帕瑞昔布钠 对比研究 

分 类 号：R-332[医药卫生] R614R730.5