潜在非小细胞肺癌标记物CHCHD2的表达及临床意义  

Value of CHCHD2 as a potential marker of non-small cell lung cancer:analysis of 60 cases

作　　者：徐锐 王胜昱[2] 王莉[3] 李满祥[1] 姚杨[3] XU Rui;WANG Shengyu;WANG Li;LI Manxiang;YAO Yang(Department of Respiratory Medicine, First Affiliated Hospital of Xi＇an Jiaotong University, Xi＇an 710061, China;Department of Respiratory Medicine;Department of Scientific Research, First Affiliated Hospital of Xi ＇an Medical University, Xi＇an 710061, China)

机构地区：[1]西安交通大学第一附属医院呼吸内科 [2]西安医学院第一附属医院呼吸内科,陕西西安710061 [3]西安医学院第一附属医院科研科,陕西西安710061

出　　处：《南方医科大学学报》2018年第3期329-333,共5页Journal of Southern Medical University

基　　金：陕西省重点研发计划项目(2017SF-336)

摘　　要：目的研究一种潜在的肿瘤标记物CHCHD2在非小细胞肺癌(NSCLC)患者肿瘤组织和癌旁组织中的表达情况。方法采用免疫组化法检测60例NSCLC患者肿瘤组织及35例癌旁组织中CHCHD2蛋白的定位与表达,分析其与肺癌患者临床病理参数、总生存(OS)之间的关系。运用Oncomine数据库分析CHCHD2m RNA在非小细胞肺癌中表达水平。结果免疫组化结果显示CHCHD2在NSCLC中的阳性表达率(75.0%)明显高于癌旁组织(17.1%),且与淋巴结转移、TNM分期、肿瘤分化程度密切相关,而与性别、年龄、组织学分型无关;Oncomine数据库分析结果表明与正常对照组相比,CHCHD2 m RNA在非小细胞肺癌中高表达(P<0.05);Kaplan-Meier生存曲线分析表明,在NSCLC中CHCHD2蛋白阳性表达组患者术后总的生存时间明显低于阴性组患者,log-rank分析其差异有统计学意义。结论 CHCHD2在NSCLC中高表达,可能作为一种潜在的肿瘤标志物参与了NSCLC的发生、发展,并促进其侵袭和转移,有望作为NSCLC早期诊断及判断不良预后的指标。Objective To investigate the expression of CHCHD2, a potential tumor marker, in tumor and adjacent tissues from patients with non-small cell lung cancer（NSCLC）. Methods Immunohistochemistry was used to detect the expression and location of CHCHD2 in the tumor tissues from 60 patients with NSCLC and 35 adjacent tissues to analyze the correlation of CHCHD2 expression with the clinicopathological variables and overall survival of the patients. The expression profile of CHCHD2 m RNA in NSCLC was analyzed using Oncomine database. Results The positivity rate of CHCHD2 was significantly higher in the tumor tissues than in adjacent tissues in patients with NSCLC（75.0% vs 17.1%）. CHCHD2 positivity in the tumor tissues was associated with lymph node metastasis, pathological TNM stage, and tumor grades but not with age, gender, or histological type of the tumors. Analysis using Oncomine database showed that CHCHD2 m RNA was expressed at significantly higher levels in NSCLC than in normal control group（P〈0.05）. Kaplan-Meier survival analysis showed that NSCLC patients with a positive expression of CHCHD2 had a significantly shorter overall survival time than those negative for CHCHD2（P〈0.05）. Conclusion As a potential tumor marker, CHCHD2 over-expression plays a role in the occurrence and progression of NSCLC and promotes tumor invasion and metastasis, and can potentially serve as an indicator for early diagnosis and prognostic evaluation of NSCLC.

关 键 词：非小细胞肺癌 CHCHD2 免疫组织化学 预后 

分 类 号：R734.2[医药卫生—肿瘤]