基于多指标成分含量与抗氧化活性的葛根类药材质量评价研究  

Quality evaluation study of Pueraria Radix and Pueraria thomsonii by component contents and anti-oxidant activity

作　　者：黄再强 王甜甜[1] 马逾英[1] 张燕飞[1] 胡明勋[1] 吴兵[1] HUANG Zai-qiang;WANG Tian-tian;MA Yu-ying;ZHANG Yan-fei;HU Ming-xun;WU Bing(College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China;College of Sanqi, Wenshan College, Wenshan 663000, China)

机构地区：[1]成都中医药大学药学院,四川成都611137 [2]文山学院三七学院,云南文山663000

出　　处：《中草药》2018年第7期1667-1676,共10页Chinese Traditional and Herbal Drugs

基　　金：四川省科技计划项目(2014SZ0131)

摘　　要：目的通过测定葛根类药材中3′-羟基葛根素、葛根素、大豆苷、大豆苷元、染料木苷、染料木素6种成分含量,结合抗氧化活性评价,综合评价葛根类药材质量。方法采用UPLC-DAD法测定样品的主要成分含量,Agi Lent Zorbax SB-C18(5 mm×2.1 mm,1.8μm),以乙腈-0.1%乙酸水溶液作为流动相洗脱,柱温30℃,体积流量0.4 mL/min;采用紫外分光光度法,以DPPH清除活性进行抗氧化活性评价;以样品中3′-羟基葛根素、葛根素、大豆苷、大豆苷元、染料木苷、染料木素6种成分含量,结合其半数抑制浓度(IC_(50)),进行7个指标的综合加权评分,并进行聚类分析。结果粉葛以8、9、50、51号样品综合加权评分较高,质量较好,产自四川简阳、江油地区;19、20、29~31号人工种植峨眉葛样品质量较差;葛根以33~37、65~69号样品综合加权评分较高,质量较好,产自平武、北川、青川地区。结论 UPLC波长切换技术多成分同时测定方法操作简单、简便快捷、重复性好,专属性强;综合加权评价法能够较全面地分析药材质量,为葛根类药材质量综合评价提供参考依据。Objective To determine the components of 3′-hydroxy puerarin, puerarin, daidzin, daidzein, genistein glycosides, and genistein from Pueraria Radix and to study the quality of medicinal materials combining with anti-oxidant activity. Methods The contents of six main components from Pueraria Radix samples were determined by using UPLC-DAD method, which performed on Agi Lent Zorbax SB-C18（5 mm × 2.1 mm, 1.8 μm）, acetonitrile and 0.1% acetic acid aqueous solution as mobile phase, column temperature 30 ℃, and the flow rate of 0.4 mL/min. The anti-oxidant activity evaluation of DPPH was accomplished by using UV spectrophotometry. Comprehensive weighted evaluation was based on seven indicators, combined six kinds of ingredients with the IC（50） of medicine, and made cluster analysis. Results Studies had shown that the quality of No. 8, 9, 50, and 51 samples of Pueraria thomsonii was better than others, which collected from Jianyang and Jiangyou. And the quality of No. 19, 20, 29, 30, and 31 samples of cultivated-planting Pueraria omeiensis was poor. The quality of No. 33 - 37, 65 - 69 samples of Pueraria Radix was better than other places in Sichuan Province, which collected from Pingwu, Beichuan, and Qingchuan. Conclusion The UPLC wavelength switching technology multicomponent simultaneous determination method is simple and convenient, which has good reproducibility, strong specificity. Comprehensive weighted evaluation method can analyze the quality of medicinal materials better, which provides references for comprehensive evaluation on the main varieties of Pueraria.

关 键 词：葛根 粉葛 UPLC-DAD 3′-羟基葛根素 葛根素 大豆苷 大豆苷元 染料木苷 染料木素 抗氧化活性 质量评价 

分 类 号：R286.2[医药卫生—中药学]