应用系统生物学方法从次生代谢物作用蛋白受体角度探索吴茱萸的归经研究  

Study on meridian tropism of Evodiae Fructus based on receptors of its secondary metabolites using system biological method

作　　者：滕杰[1] 梁怡红[2] 李毅[2] 王树青[1] 赵雅杰 谭丽楠 刘昌孝[4] TENG Jie;LIANG Yi-hong;LI Yi;WANG Shu-qing;ZHAO Ya-jie;TAN Li-nan;LIU Chang-xiao(Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, Tianjin 300070, China;People＇s Hospital of Rizhao, Rizhao 276800, China;Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China)

机构地区：[1]天津市临床药物关键技术重点实验室天津医科大学药学院,天津300070 [2]日照市人民医院,山东日照276800 [3]天津医科大学药学院 [4]天津药物研究院,天津300193

出　　处：《中草药》2018年第8期1841-1846,共6页Chinese Traditional and Herbal Drugs

基　　金：中国博士后科学基金资助项目(20110490793,2013T60260)

摘　　要：目的以吴茱萸的归经研究为例,构建基于系统生物学从次生代谢物作用蛋白受体角度研究中药归经的新方法。方法建立吴茱萸的次生代谢物成分-作用受体的复杂网络,以至少与5个化合物高度选择为条件选取蛋白受体,并将受体组织器官分布、功能与吴茱萸归经脏腑循经器官与功效互相比较应证。结果共获得吴茱萸的次生代谢物高度选择作用的34个受体,其分布和功能与吴茱萸传统归经的循经解剖器官组织归位及功效高度一致。结论应用本方法从成分作用受体角度说明中药归经的现代科学物质基础及机制,将助推归经相关研究,具有重要的参考价值。Objective Using meridian tropism of Evodiae Fructus as an example, a new method was built based on system biological method to study the tropism of Chinese herbal medicine from the view of secondary metabolites acting with protein receptors. MethodsAfter establishing the complex secondary metabolites compounds-receptors network of Evodiae Fructus, the receptors connecting with at least five compounds were selected. These functions and tissue distribution of receptors were compared with the traditional efficacy and meridian viscera and organs of Evodiae Fructus. Results A total of 34 receptors of secondary metabolites of Evodiae Fructuswere acquired. Their functions and distributions were consistent highly with the traditional efficacy and meridian tropism of Evodiae Fructus locating anatomical organs and tissues. Conclusion This original innovation method clearly elucidated the modern scientific material basis and mechanism of the meridian tropism of Chinese herbal medicine from the aspect of component action receptor. Also it will be of important reference value for the study of promoting meridian tropism.

关 键 词：吴茱萸 归经 受体 次生代谢物 系统生物学 

分 类 号：R285.5[医药卫生—中药学]