阴沟肠杆菌和大肠杆菌的生物化工发酵能力比较研究  

Comparative Study on Biochemical Fermentation Ability of Enterobacter cloacae and Escherichia coli

作　　者：汤宏赤 唐婷婷 闭德武 尹金阳 郭媛[2] 林丽华[1,2] 严少敏[2] 杜丽琴[1] 庞浩[1,2] Tang Hongchi;Tang Tingting;Bi Dewu;Yi Jinyang;Guo Yuan;Lin Lihua;Yan Shaomin;Du Liqin;Pang Hao(College of Life Science and Technology, Guangxi University, Nanning, 530004;National Engineering Research Center for Non-Food Biorefinery, State Key Laboratory of Non-Food Biomass and Enzyme Technology, Guangxi Key Laboratory of Bio-refinery, Guangxi Academy of Sciences, 98 Daling Road, Nanning, 530007, China)

机构地区：[1]广西大学生命科学与技术学院,南宁530004 [2]国家非粮生物质能源工程技术研究中心非粮生物质酶解国家重点实验室广西生物炼制重点实验室广西科学院,南宁530007

出　　处：《基因组学与应用生物学》2018年第4期1519-1525,共7页Genomics and Applied Biology

基　　金：国家自然科学基金(21366007);广西自然科学基金(2014GXNSFAA118078);广西科技基地和人才专项(桂科AD16380016);广西科技攻关(桂科合1347004-1)共同资助

摘　　要：本研究通过对Escherichia coli JM109菌株和E.cloacae gxas菌株的碳源利用能力、菌株的生长优势以及胞内发酵产物组成进行了研究。实验结果表明：在碳源利用测试中E.coli JM109结果表现阳性（＋）只有8种,阴性（-）有32种,而E.cloacae gxas阳性（＋）有38种,阴性（-）只有18种,具有广泛的碳源谱。比较2株菌株的生长曲线发现E.cloacae gxas比E.coli JM109生长快,稳定期具有更高的生物量。GC-MS分析结果显示两个菌株的发酵产物的差异极大,在多尺度面上没有重合点。以大肠杆菌为对照菌株,阴沟肠杆菌的发酵产物中有14 776个化合物的产量比大肠杆菌高,只有7 725个化合物的产量比大肠杆菌低。因而,阴沟肠杆菌在生物化工发酵应用中比大肠杆菌更具潜力优势。In this study, the ability of carbon source utilization of Escherichia coli JM109 and E. cloacae gxas, the growth advantage of strains and the composition of intracellular fermentation products were studied. The results showed that there were only 8 positive and 32 negative in E. coli JM109 by the carbon source utilization tests, but there were 38 positive and 18 negative in E. cloacae gxas. Thus, E. cloacae showed a wide spectrum of carbon source.Comparing the growth curves of these two strains, it was found that the growth rate of E. cloacae gxas was faster than that of E. coli JM109. Besides, the biomass of E. cloacae gxas was also higher than that of E. coli JM109 in stable period. The results of GC-MS analysis showed that two strains had greatly different fermentation products. There was no coincident point in multi-scale analysis. Using E. coli as control strain, in the ferm entation products of E.cloacae, the yield of 14 776 compounds was higher than that of E. coli, and only 7 725 compound yield was lower than that of E. coli. Therefore, E. cloacae strain might have more potential advantages than E. coli strain in the biochemical fermentation application.

关 键 词：阴沟肠杆菌 碳源利用 GC-MS 生物化工发酵 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]