蓬莪术根茎总黄酮的提取及体外抗氧化分析  

Extraction and in vitro Antioxidant Analysis of Total Flavonoids from Curcuma phaeocaulis Rhizomes

作　　者：李洋益 高刚[2] 张艳[1] 邓家彬[3] 童珊珊[1] 张仲豪 杨瑞武[1] Li Yangyi;Gao Gang;Zhang Yan;Deng Jiabin;Tong Shanshan;Zhang Zhonghao;Yang Ruiwu(College of Life Science, Sichuan Agricultural University, Ya＇an, 625014;Life Science and Food Engineering College, Yibin University, Yibin, 644000;School of Geography and Tourism, Guizhou Normal College, Guiyang, 550018)

机构地区：[1]四川农业大学生命科学学院,雅安625014 [2]宜宾学院生命科学与食品工程学院,宜宾644000 [3]贵州师范学院地理与旅游学院,贵阳550018

出　　处：《基因组学与应用生物学》2018年第4期1614-1620,共7页Genomics and Applied Biology

基　　金：国家自然科学基金(31270243)资助

摘　　要：为了合理地开发和利用蓬莪术的药用价值,通过响应面法对蓬莪术根茎总黄酮超声波辅助提取进行工艺优化,并探究粗制黄酮和纯化黄酮的还原力和对DPPH、羟基自由基、超氧阴离子的清除能力。结果表明,在超声功率300 W的条件下,以乙醇浓度80%、液料比40 mL/g、超声时间50 min、超声温度70℃的提取效果最佳,总黄酮得率为1.593%。在体外抗氧化活性分析中,蓬莪术根茎粗制黄酮对DPPH、羟基自由基、超氧阴离子及还原力的IC50值分别为1.255 mg/mL、0.597 mg/mL、1.041 mg/mL和3.508 mg/mL,而纯化黄酮的IC50值分别为0.333 mg/mL、0.076 mg/mL、0.084 mg/mL和1.477 mg/mL。因此,响应面可以很好的对蓬莪术根茎总黄酮超声辅助提取进行优化,且粗制黄酮和纯化黄酮都具有良好的抗氧化活性。In order to reasonably develop and utilize the medicinal value of Curcuma phaeocaulis, the technique of ultrasonic-assisted extraction of total flavonoids from Curcuma phaeocaulis rhizomes was optimized by response surface method, and the reducing power of purified and unpurified flavonoids as well as the scavenging activities on DPPH, hydroxyl radical and superoxide anion were analyzed. The results showed that under the condition of 300 W ultrasonic power, the optimum extraction effects could be achieved at 80% ethanol concentration, 40 m L/g liquid-to-solid ratio, 50 min ultrasonic time, 70℃ ultrasonic temperature, and the extraction rate of total flavonoids was 1.593%. In the in vitro antioxidant analysis, the scavenging activities on DPPH, hydroxyl radical, and superoxide anion as well as the reducing power were tested. the IC50 values of the unpurified flavonoids extracted from C. phaeocaulis rhizomes were 1.255 mg/m L, 0.597 mg/m L, 1.041 mg/m L and 3.508 mg/m L, respectively, while the IC50 values of the purified flavon oids extracted were 0.333 mg/m L,0.076 mg/m L, 0.084 mg/m L and 1.477 mg/m L, respectively. Therefore, response surface method could optimize the ultrasonic-assisted extraction of total flavonoids from C. phaeocaulis rhizomes, and the purified and unpurified flavonoids both had excellent antioxidant activity.

关 键 词：蓬莪术 黄酮化合物 响应面 体外抗氧化 

分 类 号：R284.2[医药卫生—中药学] R285[医药卫生—中医学]