斑蝥酸钠注射液可有效降低炎性因子与血管内皮生长因子的表达  

Disodium Cantharide Injection can Effectively Reduce the Expression of Inflammatory Factors and Vascular Endothelial Growth Factor

作　　者：单福新 沙春艳[1] Shan Fuxin;Sha Chunyan(Baicheng Medical College, Baicheng, 137000)

机构地区：[1]白城医学高等专科学校

出　　处：《基因组学与应用生物学》2018年第4期1685-1690,共6页Genomics and Applied Biology

基　　金：白城医学高等专科学校对本研究的资助

摘　　要：为了探究斑蝥酸钠注射液对胃癌前病变大鼠炎性因子与血管内皮生长因子的影响,我们选取了36只SD大鼠（美国Sprague Dawley农场用Wistar大鼠培育而成）,建立了大鼠胃黏膜癌前病变模型,采用随机数字方法分为3组,每组12只。其中,我们对斑蝥酸钠组采用斑蝥酸钠治疗,5-氟尿嘧啶（5-fluoro-2,4（1h,3h）pyrimidinedione,5-FU）5-FU组采用5-FU药物治疗,生理盐水组注射生理盐水治疗,并采用酶联免疫吸附实验检测了肿瘤坏死因子α（tumor necrosis factorα,TNF-α）、白细胞介素-8（interleukin 8,IL-8）及C反应蛋白（C-reactive protein,CRP）的表达水平;采用免疫组化法及半定量方法比较了三组肿瘤组织中的血管内皮生长因子（vascular endothelial growth factor,VEGF）VEGF-C、VEGFR-3蛋白的表达。我们发现,斑蝥酸钠组处理后,TNF-α、IL-8及CRP水平低于5-FU组和生理盐水组（p〈0.05）;5-FU组处理后,TNF-α、IL-8及CRP水平低于生理盐水组（p〈0.05）;斑蝥酸钠组大鼠处理后VEGF-C、VEGFR-3蛋白表达水平低于5-FU组和生理盐水组（p〈0.05）;5-FU组大鼠处理后,VEGF-C、VEGFR-3蛋白表达水平低于生理盐水组（p〈0.05）。本研究表明大鼠胃黏膜病变利用斑蝥酸钠注射液治疗效果理想,具有广泛的运用前景。我们的研究为胃黏膜癌前病变的治疗及机制的研究提供了一定的帮助。To investigate the effects of disodium cantharidinate injection on inflammatory factors and vascular endothelial growth factor in gastric precancerous lesions in rats, we selected 36 SD rats（cultured from Wistar rats in Sprague Dawley farms） to establish a precancerous lesion model of gastric mucosa in rats. They were divided into three groups by random number method, with 12 rats in each group. Disodium cantharidinate group was treated with disodium cantharidinate, 5-fluoro-2,4（1 h, 3 h） pyrimidinedione（5-FU） group was treated with 5-FU,and normal saline group was injected with normal saline. Enzyme-linked immunosorbent assay was used to detect expressionlevelsoftumornecrosisfactorα（tumor, necrosis, factor α, TNF-α）, interleukin-8（IL-8） and C reactive protein（CRP）.Theexpressionsofvascularendothelialgrowthfactor（VEGF）,VEGF-Cand VEGFR-3 proteinsinthree groups of tumor tissues were compared by immunohistochemistry and semi quantitative methods. We found that the levels of TNF-α, IL-8 and CRP in disodium cantharidinate group were lower than those in 5-FU and normal saline group（p〈0.05）. TNF-α, IL-8 and CRP levels in 5-FU group were lower than those in normal saline group（p〈0.05）. Afterthetreatmentofdisodiumcantharidinate,theexpressionlevelof VEGF-Cand VEGFR-3 protein was lower than that in 5-FU and normal saline group（p〈0.05）. The expression level of VEGF-C and VEGFR-3 in5-FU group was lower than that in normal saline group（p〈0.05）. This study indicated that the treatment of gastric mucosal lesions in rats using disodium cantharidinate injection was ideal and had broad application prospects. Our researchcouldprovidecertainhelpforthetreatmentandmechanismofprecancerouslesionofgastricmucosa.

关 键 词：斑蝥酸钠注射液 胃癌前病变 炎症因子 血管内皮生长因子 酶联免疫吸附实验 

分 类 号：R735.2[医药卫生—肿瘤]