Nanocapsules of oxalate oxidase for hyperoxaluria treatment  

作　　者：Ming Zhao Duo Xu Di Wu James W. Whittaker Robert Terkeltaub Yunfeng Lu 

机构地区：[1]Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, Los Angeles CA 90095, USA [2]Division of Environmental and Biomolecular Systems, Oregon Health and Sciences University, Beaverton OR 97006-8921, USA [3]Division of Rheumatology, Allergy and Immunology, San Diego VA Medical Center, 3350 La Jolla Village Drive, San Diego CA 92161, USA

出　　处：《Nano Research》2018年第5期2682-2688,共7页纳米研究（英文版）

摘　　要：Enzyme therapeutics have great potential for the treatment of systemic disorders such as urolithiasis and nephrocalcinosis, which are caused by the excessive accumulation of oxalate. However, exogenous enzymes have short half-lives in vivo and elicit high immunogenicity, which largely limit the therapeutic outcomes. Herein, we report a delivery strategy whereby therapeutic enzymes are encapsulated within a thin zwitterionic polymer shell to form enzyme nanocapsules. The strategy is exemplified by the encapsulation of oxalate oxidase （OxO） for the treatment of hyperoxaluria, because as-synthesized OxO nanocapsules have a prolonged blood circulation half-life and elicit reduced immunogenicity. Our design of enzyme nanocapsules that enable the systemic delivery of therapeutic enzymes can be extended to various biomedical applications.Enzyme therapeutics have great potential for the treatment of systemic disorders such as urolithiasis and nephrocalcinosis, which are caused by the excessive accumulation of oxalate. However, exogenous enzymes have short half-lives in vivo and elicit high immunogenicity, which largely limit the therapeutic outcomes. Herein, we report a delivery strategy whereby therapeutic enzymes are encapsulated within a thin zwitterionic polymer shell to form enzyme nanocapsules. The strategy is exemplified by the encapsulation of oxalate oxidase （OxO） for the treatment of hyperoxaluria, because as-synthesized OxO nanocapsules have a prolonged blood circulation half-life and elicit reduced immunogenicity. Our design of enzyme nanocapsules that enable the systemic delivery of therapeutic enzymes can be extended to various biomedical applications.

关 键 词：enzyme therapeutics HYPEROXALURIA oxalate oxidase protein delivery long circulation NANOMEDICINE 

分 类 号：TE254.4[石油与天然气工程—油气井工程] TS312[轻工技术与工程]