Response of ammonia-oxidizing betaproteobacteria to short- term fertilization in a salt marsh in China  

作　　者：MA Yuexin TAO Wei LIU Jiao LIU Changfa LI Jin LIU Jichen 马悦欣;陶韦;刘姣;刘长发;李晋;刘继晨(Key Laboratory of Mariculture and Stock Enhancement in North China＇s Sea of Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China;Key Laboratory of Marine Environmental Research of Liaoning Higher Education, Dalian 116023, China;Institute of Ocean and Fisheries ofPanjin, Panjin 124001, China)

机构地区：[1]Key Laboratory of Mariculture and Stock Enhancement in North China's Sea of Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China [2]Key Laboratory of Marine Environmental Research of Liaoning Higher Education, Dalian 116023, China [3]Institute of Ocean and Fisheries of Panjin, Panjin 124001, China

出　　处：《Journal of Oceanology and Limnology》2018年第2期351-361,共11页海洋湖沼学报（英文）

基　　金：Supported by the National Natural Science Foundation of China(No.41171389);the Public Science and Technology Research Funds Projects of Ocean(No.201305043);the Program for Liaoning Excellent Talents in University(No.LR2013035)

摘　　要：This study examines the impacts of short-term(6 months) fertilization on the community structure and abundance of ammonia-oxidizing betaproteobacteria(β-AOB) and the potential nitrification rate in sediment colonized by S uaeda heteroptera in a saltmarsh located in Shuangtai estuary, China. The sediment samples were collected from plots treated with different amounts of an N fertilizer(urea supplied at 0.1, 0.2, 0.4, and 0.8 g/kg(nitrogen content in dry sediment)), and with different forms of N fertilizers(urea,(NH4) 2 SO 4, and NH_4NO_3, each supplied at 0.2 g/kg). The fertilizers were applied 1–4 times during the plant-growing season in May, July, August and September of 2013. Untreated plots were included as a control. As revealed in denaturing gradient gel electrophoresis of the 16 S r RNA gene, the β-AOB community responded to both the amount and form of N. Real-time quantitative PCR indicated that both abundance and potential nitrification rate of β-AOB increased after N addition, regardless of concentration and form(except NH_4NO_3). These results provide evidence that short-term N application influences the sediment β-AOB community, β-AOB abundance and potential nitrification rate in a saltmarsh ecosystem.This study examines the impacts of short-term （6 months） fertilization on the community structure and abundance of ammonia-oxidizing betaproteobacteria （β-AOB） and the potential nitrification rate in sediment colonized by Suaeda heteroptera in a saltmarsh located in Shuangtai estuary, China. The sediment samples were collected from plots treated with different amounts of an N fertilizer （urea supplied at 0.1, 0.2, 0.4, and 0.8 g/kg （nitrogen content in dry sediment））, and with different forms of N fertilizers （urea, （NH4）2SO4, and NH4NO3, each supplied at 0.2 g/kg）. The fertilizers were applied 1-4 times during the plant-growing season in May, July, August and September of 2013. Untreated plots were included as a control. As revealed in denaturing gradient gel electrophoresis of the 16S rRNA gene, the β-AOB community responded to both the amount and form of N. Real-time quantitative PCR indicated that both abundance and potential nitrification rate of β-AOB increased after N addition, regardless of concentration and form （except NH4NO3）. These results provide evidence that short-term N application influences the sediment β-AOB community, β-AOB abundance and potential nitrification rate in a saltmarsh ecosystem.

关 键 词：ammonia-oxidizing betaproteobacteria (β-AOB) denaturing gradient gel electrophoresis (DGGE) nitrification FERTILIZATION SALTMARSH 

分 类 号：P[天文地球]