Intestinal microbiota of healthy and unhealthy Atlantic salmon Salmo salar L. in a recirculating aquaculture system  

作　　者：WANG Chun SUN Guoxiang LI Shuangshuang LI Xian LIU Ying 王纯;孙国祥;李双双;李贤;刘鹰(Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;University of Chinese Academy of Sciences, Beijing 100049, China;College of Energy and Environmental Engineering, Hebei University of Engineering, Handan 056038, China)

机构地区：[1]Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China [2]University of Chinese Academy of Sciences, Beijing 100049, China [3]College of Energy and Environmental Engineering, Hebei University of Engineering, Handan 056038, China

出　　处：《Journal of Oceanology and Limnology》2018年第2期414-426,共13页海洋湖沼学报（英文）

基　　金：Supported by the National Natural Science Foundation of China(Nos.31472312,41306152,31402283);the Qingdao Innovation Talents Program(No.13-CX-16);the National Key Technology Research and Development Program of China(No.2014BAD08B09);the Earmarked Fund for Modern Agroindustry Technology Research System(No.CARS-48);the Project for International S&T Cooperation Program of China(No.2014DFA31030)

摘　　要：The present study sampled the intestinal content of healthy and unhealthy Atlantic salmon （Salmo salar L.）, the ambient water of unhealthy fish, and the biofilter material in the recirculating aquaculture system （RAS） to understand differences in the intestinal microbiota. The V4--V5 regions of the prokaryotic 16S rRNA genes in the samples were analyzed by MiSeq high-throughput sequencing. The fish were adults with no differences in body length or weight. Representative members of the intestinal microbiota were identified. The intestinal microbiota of the healthy fish included Proteobacteria （44.33%）, Actinobacteria （17.89%）, Baeteroidetes （15.25%）, and Firmicutes （9.11%）, among which the families Mierococcaceae and Oxalobacteraceae and genera Sphingomonas, Streptomyces, Pedobacter, Janthinobacterium, Burkholderia, and Balneimonas were most abundant. Proteobacteria （70.46%）, Bacteroidetes （7.59%）, and Firmicutes （7.55%） dominated the microbiota of unhealthy fish, and Chloroflexi （2.71%）, and Aliivibrio and Vibrio as well as genera in the family Aeromonadaceae were most strongly represented. Overall, the intestinal hindgut microbiota differed between healthy and unhealthy fish. This study offers a useful tool for monitoring the health status of fish and for screening the utility of probiotics by studying the intestinal microbiota.The present study sampled the intestinal content of healthy and unhealthy Atlantic salmon( Salmo salar L.), the ambient water of unhealthy fish, and the biofilter material in the recirculating aquaculture system(RAS) to understand differences in the intestinal microbiota. The V4–V5 regions of the prokaryotic 16 S r RNA genes in the samples were analyzed by Mi Seq high-throughput sequencing. The fish were adults with no differences in body length or weight. Representative members of the intestinal microbiota were identified. The intestinal microbiota of the healthy fishincluded Proteobacteria(44.33%), Actinobacteria(17.89%), Bacteroidetes(15.25%), and Firmicutes(9.11%), among which the families Micrococcaceae and Oxalobacteraceae and genera S phingomonas, Streptomyces, Pedobacter, Janthinobacterium, Burkholderia, and Balneimonas were most abundant. Proteobacteria(70.46%), Bacteroidetes(7.59%), and Firmicutes(7.55%) dominated the microbiota of unhealthy fish, and Chloroflexi(2.71%), and Aliivibrio and Vibrio as well as genera in the family Aeromonadaceae were most strongly represented. Overall, the intestinal hindgut microbiota differed between healthy and unhealthy fish. This study off ers a useful tool for monitoring the health status of fish and for screening the utility of probiotics by studying the intestinal microbiota.

关 键 词：intestinal microbiota health status Atlantic salmon(Salmo salar L.) recirculating aquaculture system high-throughput pyrosequencing 

分 类 号：P[天文地球]