Comparative analysis on microbial community associated with different gastrointestinal regions of wild northern snakehead Channa argus Cantor, 1842  

作　　者：MIAO Shuyan ZHAO Chenze ZHU Jinyu PAN Mingzhu 苗淑彦;赵臣泽;朱锦裕;潘明珠(College of Animal Science and Technology, Yangzhou University)

机构地区：College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

出　　处：《Journal of Oceanology and Limnology》2018年第2期450-456,共7页海洋湖沼学报（英文）

基　　金：Supported by the National Natural Science Foundation of China(No.31402306)

摘　　要：Microbial communities in different gastrointestinal regions （stomach, foregut, midgut, and hindgut） of the northern snakehead Channa argus （Cantor, 1842） were compared by polymerase chain reaction and partial 16S rDNA sequencing. A total of 194, 140, 212, and 122 OTUs were detected in the stomach, foregut, midgut, and hindgut, respectively. Significant differences were found in the Sobs, ACE, Shannon, and Simpson indices among samples （P〈0.05）. The gastrointestinal microbial community of C. argus consisted predominantly of Proteobacteria with either Halomonas, Shewanella, Plesiomonas, or Sphingomonas. Fusobacteria, Firm!cutes, and Bacteroidetes also existed in the gastrointestinal tracts. However, significant＇differences were found in the compositions of microbial community among the four regions （P〈0.05）. Cyanobacteria and Spirochetes were significantly higher in the midgut and hindgut （P〈0.05）. Fusobacteria and Firmicutes were dominant in the hindgut and foregut, respectively （P〈0.05）. Proteobacteria was the lowest in the hindgut （P〈0.05）. At genus level, Cetobacterium and Plesiomonas were significantly higher in the hindgut than in the other three samples （P〈0.05）. Clostridium and Prevotella were the highest in the midgut （P〈0.05）. Halomonas, Shewanella, and Sphingomonas were the highest in the foregut （P〈0.05）. Paracoccus and Vibrio were the highest in the stomach. Several genera were only detected in certain regions, as follows： stomach, Paracoccus and Hbrio; foregut, Halomonas, Shewanella, and Sphingomonas; midgut, Clostridium and Prevotella; and hindgut, Cetobacterium and Plesiomonas （P〈0.05）. At the species level, Acinetobacter rhizosphaerae was only detected in the stomach. Prevotella copri and Clostridium perfring were not detected in the foregut and midgut, respectively, whereas Prevotella copri and Faecalibacterium pra were not detected in the hindgut. These findings provide valuable information on the microbial community in each gastroMicrobial communities in different gastrointestinal regions(stomach, foregut, midgut, and hindgut) of the northern snakehead C hanna argus(Cantor, 1842) were compared by polymerase chain reaction and partial 16 S r DNA sequencing. A total of 194, 140, 212, and 122 OTUs were detected in the stomach, foregut, midgut, and hindgut, respectively. Significant dif ferences were found in the Sobs, ACE, Shannon, and Simpson indices among samples(P <0.05). The gastrointestinal microbial community of C. argus consisted predominantly of Proteobacteria with either H alomonas, Shewanella, Plesiomonas, or Sphingomonas. Fusobacteria, Firmicutes, and Bacteroidetes also existed in the gastrointestinal tracts. However, significant diff erences were found in the compositions of microbial community among the four regions( P <0.05). Cyanobacteria and Spirochetes were significantly higher in the midgut and hindgut( P <0.05). Fusobacteria and Firmicutes were dominant in the hindgut and foregut, respectively(P <0.05). Proteobacteria was the lowest in the hindgut( P <0.05). At genus level, C etobacterium and Plesiomonas were significantly higher in the hindgut than in the other three samples( P <0.05). Clostridium and Prevotella were the highest in the midgut(P <0.05). Halomonas, Shewanella, and S phingomonas were the highest in the foregut( P <0.05). Paracoccus and Vibrio were the highest in the stomach. Several genera were only detected in certain regions, as follows: stomach, P aracoccus and Vibrio; foregut, Halomonas, Shewanella, and Sphingomonas; midgut, Clostridium and Prevotella; and hindgut, C etobacterium and Plesiomonas( P <0.05). At the species level, A cinetobacter rhizosphaerae was only detected in the stomach. P revotella copri and Clostridium perfring were not detected in the foregut and midgut, respectively, whereas Prevotella copri and Faecalibacterium pra were not detected in the hindgut. These findings provide valuable information on the microbial community in each gastrointestinal region of C. argus. Moreover, this stu

关 键 词：northern snakehead (Channa argus Cantor  1842) 16S rDNA microbial community gastrointestinal region physico-chemical characteristic 

分 类 号：P[天文地球]