LASP-1对不同来源成骨细胞的运动、成骨功能及细胞周期分布的影响  

Impacts of LASP-1 on osteogenic moving behavior and distribution of cell cycle of osteoblasts derived from different source

作　　者：贾芳 赖春花 周震 高岩 郭泽鸿 徐淑兰 JIA Fang;LAI Chunhua;ZHOU Zhen;GAO Yan;GUO Zehong;XU Shulan(Planting Center,Stomatology Hospital of Southern Medical University,Guangzhou 510082,China)

机构地区：[1]南方医科大学口腔医院,广州510082

出　　处：《实用医学杂志》2018年第9期1428-1434,共7页The Journal of Practical Medicine

基　　金：广东省医学科研基金(编号:A2015589);广东省科技厅科技计划项目(编号:[2015]110号)

摘　　要：目的研究LASP-1是否影响肿瘤来源成骨样细胞和非肿瘤来源成骨细胞的运动、成骨、增殖能力以及细胞周期的分布比例。方法采用qRT-PCR及Western Blot(WB)检测小鼠骨髓间充质干细胞(BMMSC)、人成骨细胞(h OB)以及人骨肉瘤细胞(MG-63)中LASP-1的表达;通过构建重组载体,转染sh-LASP-1,检测各细胞中LASP-1表达的变化,采用划痕实验、XTT法、WB、流式细胞仪检测该变化对细胞运动能力、骨钙素(OCN)表达、细胞增殖能力和细胞周期分布比例的影响。结果上调或下调LASP-1的表达,非肿瘤来源细胞(BMMSC,h OB)的运动能力、骨钙素(OCN)的表达、细胞增殖能力及G2/M期的细胞比例未见明显改变;对肿瘤来源细胞(MG-63)而言,随LASP-1的表达上调,OCN表达亦上调,细胞数量增多,且处于G2/M期的细胞比例降低;对各细胞运动能力未见显著影响。结论 LASP-1对非肿瘤来源细胞的成骨性能、增殖能力以及细胞周期分布比例未见明显影响;而对肿瘤来源细胞(MG-63)有明显调节作用。Objective To investigate the impacts of LASP-1 on the behavior and cell proliferation abilityof tumor derived osteoblast-like cells and non tumor derived osteoblasts. Methods QRT-PCR and Western blotwere performed to detect LASP-1 expressions in mouse bone marrow mesenchymal stem cells（BMMSC）,humanosteoblasts（h OB）and human osteosarcoma cell（MG-63）. With the construction of recombinant vector and thetransfection of sh LASP-1,the expression changes in both LASP-1 and osteocalcin（OCN）,and the cell number ofcell-cycle phase distribution were analyzed. Results With up-or down-regulation of LASP-1,osteocalcin（OCN）expression,cell proliferation ability and the cell number of G2/M phase in non tumor cells（BMMSC,h OB）hadno obvious changes. However,as for the tumor cells（MG-63）,with an increased expression of LASP-1,the ex-pression of OCN,cell proliferation number and the cell number at G2/M phase changed. There was no significanteffect on the moving ability of each cell. Conclusions LASP-1 has no significant effect on the osteogenic proper-ties,cell proliferation ability and cell cycle distribution of non tumor derived cells,but has a regulatory effect ontumor derived cells（MG-63）.

关 键 词：LASP-1 非肿瘤来源 肿瘤来源 成骨 细胞周期 

分 类 号：R730.2[医药卫生—肿瘤] R783[医药卫生—临床医学]