超声联合SDF-1微泡对糖尿病性心肌病大鼠心肌损伤的保护作用  

Protective effects of ultrasound and SDF-1 microbubbles on myocardial injury in rats with diabetic cardiomyopathy

作　　者：王琳琳[1] 李文建[2] 李易明[2] 杨漪[1] 闫凤琴[1] WANG Linlin;LI Wenjian;LI Yiming;YANG Yi;YAN Fengqin(Harrison International Peace Hospital, Hengshui 053100, Hebei, China;Hebei Medical University, Shijiazhuang 050017, Hebei, China)

机构地区：[1]哈励逊国际和平医院,河北衡水053100 [2]河北医科大学,河北石家庄050017

出　　处：《现代中西医结合杂志》2018年第14期1501-1504,共4页Modern Journal of Integrated Traditional Chinese and Western Medicine

摘　　要：目的研究超声联合基质细胞衍生因子-1(SDF-1)微泡对糖尿病性心肌病大鼠心肌损伤的保护作用。方法将40只SD大鼠随机分为空白组、模型组、SDF-1组、SDF-1+超声组、微泡+超声组,每组8只。除空白组外,其余组均采用高脂饲料喂养和腹腔注射1%链脲佐菌素(STZ)方法制备糖尿病性心肌病模型。造模成功后,SDF-1组经尾静脉输入SDF-1溶液10 ng/kg;SDF-1+超声组经尾静脉输入SDF-1溶液10ng/kg,然后采用2%戊巴比妥钠1 mL/kg腹腔注射麻醉大鼠,利用超声治疗仪辐照大鼠心前区10 min;微泡+超声组经尾静脉输入含有10 ng/kg SDF-1的微泡混合液,超声照射同SDF-1+超声组。干预4 d后,留取血清标本用于检测肌酸激酶(CK)、乳酸脱氢酶(LDH)、心肌肌钙蛋白I(cT nI)水平;干预8周后,超声心动图检测各组大鼠左心室舒张末期内径(LVIDd)、左心室舒张末压(LVEDP)和左心室射血分数(LVEF),Western blot法检测各组心肌组织中CXC族趋化因子受体4(CXCR4)蛋白表达情况,实时定量PCR法检测各组心肌组织中转化生长因子β1(TGF-β1)mR NA表达情况。结果干预4 d后,各组血清CK、LDH和cT nI水平比较差异均无统计学意义(P均>0.05)。干预8周后,各干预组LVIDd、LVEDP均明显低于模型组(P均<0.05),LVEF均明显高于模型组(P均<0.05),微泡+超声组各指标改善情况均明显优于SDF-1组和SDF-1+超声组(P均<0.05);模型组及各干预组CXCR4蛋白表达水平和TGF-β1mR NA表达水平均明显高于空白组(P均<0.05),各干预组TGF-β1mR NA表达水平均明显低于模型组(P均<0.05);微泡+超声组CXCR4蛋白表达水平明显高于其他组(P均<0.05),TGF-β1mR NA表达水平均明显低于SDF-1组和SDF-1+超声组(P均<0.05)。结论超声联合SDF-1微泡可延缓糖尿病性心肌病大鼠心肌纤维化。Objective It is to investigate the protective effects of ultrasound and stroma cell-derived factor-1（ SDF-1）microbubbles on myocardial injury in the rats with diabetic cardiomyopathy. Methods 40 SD rats were randomly divided into blank group（ group A）,model group（ group B） and SDF-1 group（ group C）,SDF-1 plus ultrasound group（ group D）,microbubbles plus ultrasound group（ group E）,each group had 8 rats. All the rats except in group A were given high fat diet and 1% STZ by intraperitoneal injection to establish diabetic cardiomyopathy models. Group B,group C,group D and group E were treated with respective therapy. After treatment for 4 days,the serum examples were collected for myocardial enzyme testing including CK,LDH,c Tn I; after treatment for 8 days,LVIDd and LVEDP and LVEF of the rats in each group were detected by echocardiography,the protein expression of CXCR4 in each group were detected by Western blot,the expression of TGF-β1 gene m RNA was detected by real-time quantitative PCR. Results After treatment for 4 days,there was no significant difference in the levels of serum CK,LDH and c Tn I among every group（ P＜0. 05）. After treatment for8 days,LVIDd,LVEDP in every treatment group were lower and LVEF was higher than that in model group（ P＜0. 05）,and the improvements in group E were better than that in group C,group D（ P＜0. 05）; the expression of CXCR4 and TGF-β1 m RNA in group B and every treatment group was higher than that in group A（ P＜0. 05）,furthermore,the expression of TGF-β1 m RNA in every treatment group was lower than that in group B（ P＜0. 05）,the expression of CXCR4 in group E was higher than that in the other treatment groups（ P＜0. 05）,that of TGF-β1 m RNA in group E was lower than that in group C and group D（ P＜0. 05）. Conclusion Ultrasound and SDF-1 microbubbles can delay myocardial fibrosis in rats with diabetic cardiomyopathy.

关 键 词：超声 微泡 基质细胞衍生因子-1 CXC族趋化因子受体4 糖尿病性心肌病 

分 类 号：R-332[医药卫生]