谷子种子DNA快速提取新方法  

A New Method for Rapid Extraction of DNA from Millet Seeds

作　　者：庞冰 王艳梅[2,3] 郝建平 王陆军[2,3] 王创云 李培良[2,3] 邓艳芳 周琼[3] 李志敏 董永军[1] 张婷婷 裴成成 牛学谦 PANG Bing;WANG Yanmei;HAO Jianping;WANG Lujun;WANG Chuangyun;LI Peiliang;DENG Yanfang;ZHOU Qiong;LI Zhimin;DONG Yongjun;ZHANG Tingting;PEI Chengcheng;NIU Xueqian(College of Life Science, Shanxi University, Taiyuan 030006, China;Institute of Crop Sciences, Shanxi Academy of AgriculturalSciences, Taiyuan 030031, China;Shanxi Corn Engineering Technology Research Center, Taiyuan 030031, China;Institute ofAgricultural Science and Technology Information, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China)

机构地区：[1]山西大学生命科学学院,山西太原030006 [2]山西省农业科学院作物科学研究所,山西太原030031 [3]山西省玉米工程技术研究中心,山西太原030031 [4]山西省农业科学院农业科技信息研究所,山西太原030031

出　　处：《山西农业科学》2018年第5期680-682,共3页Journal of Shanxi Agricultural Sciences

基　　金：山西省科技攻关项目(20140311001-8);山西省玉米工程技术研究中心建设项目(201605D141005);山西省重点研发计划项目(201703D221005-1);山西省农业科学院博士基金项目(YBSJJ1602)

摘　　要：为满足谷子高通量分子标记检测的需要,探究在不使用液氮研磨情况下,无需经过发芽,改进CTAB方法,直接从干种子中提取DNA。结果显示,采用新方法对谷子种子提取的DNA经过分光光度计以及琼脂糖电泳检测得出,A_(260/280)值在1.88~1.92,质量浓度在1 662.85~2 551.89 ng/μL;并以此为模板进行SSR-PCR扩增,结果显示,所得条带清晰、多态性丰富。该方法提取的谷子种子DNA质量稳定,能够满足分子标记检测的要求,同时简化了操作流程,实现了谷子种子DNA的高通量快速提取,为谷子高通量分子标记检测奠定了基础。To meet the need of high-throughput molecular marker detection of millet, millet seeds were used as materials to explore DNA extraction of millet seeds directly by simplified CTAB without using liquid nitrogen grinding and without germination. The results showed that the DNA extracted from the seeds of millet using the new method was detected by spectrophotometer and agarose gel electrophoresis, the A_（260/280） value was 1.88-1.92, and the mass concentration was 1 662.85-2 551.89 ng/μL. Using this as a template SSR-PCR amplification, the resulting bands was clear, polymorphisms was rich. The DNA quality of millet seeds extracted by the method is stable and can meet the requirements of molecular marker detection. At the same time, the operation process is simplified, and the high throughput and rapid extraction of DNA in millet seed is realized, which lays a foundation for high flux molecular marker detection of millet.

关 键 词：谷子 CTAB 种子 SSR-PCR 

分 类 号：S515[农业科学—作物学]