柴胡疏肝散对功能性消化不良大鼠内质网应激相关分子IRE1和TRAF2表达的影响  

Effect of Chaihushugansan decoction on expression of endoplasmic reticulum stress-related molecules IRE1 and TRAF2 in functional dyspepsia rats

作　　者：徐宽 凌江红[1,2] 周洲 张丽敏 张钰琴[1] 谢天一[1] 王煜姣[1] Xu Kuan;Ling Jianghong;Zhou Zhou(Dept of Chinese M edicine, The First Affiliated Hospital o f Guangxi Medical University, Nanning 530021;The Affiliated Zhoupu Hospital, Shanghai University o f Medicine ＆ Health Sciences, Shanghai 201318)

机构地区：[1]广西医科大学第一附属医院中医科,南宁530021 [2]上海健康医学院附属周浦医院,上海201318

出　　处：《安徽医科大学学报》2018年第4期516-520,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81560763);上海市浦东新区卫生系统重点学科建设资助(编号:PWZXK2017-02)

摘　　要：目的柴胡疏肝散对功能性消化不良(FD)大鼠胃窦组织内质网应激因子肌醇需求酶1(IRE1)、肿瘤坏死因子受体相关因子2(TRAF2)的影响。方法将30只大鼠随机均分为5组:柴胡疏肝散组、多潘立酮组、生理盐水组、模型组、正常组。正常组大鼠正常饲养,模型组只夹尾不灌药,其余组给予对应的液体灌胃。造模过程中,记录大鼠生活状态的变化。干预1个月后,测定大鼠胃排空率,免疫组织化学法观察各组IRE1、TRAF2蛋白表达情况,用逆转录PCR测定胃窦组织IRE1、TRAF2 mRNA的表达。结果生理盐水组、模型组胃排空率较正常组降低(P<0.05),柴胡疏肝散组和多潘立酮组胃排空率较模型组增高(P<0.05)。与正常组大鼠比较,模型组和生理盐水组IRE1、TRAF2蛋白水平明显上调;与模型组对比,各给药组的IRE1、TRAF2蛋白呈下调趋势(P<0.05)。RT-PCR显示柴胡疏肝散组和多潘立酮组的IRE1、TRAF2 mRNA的表达较模型组降低,而模型组IRE1、TRAF2 mRNA的表达比正常组高(P<0.05)。结论柴胡疏肝散通过抑制内质网应激分子IRE1和TRAF2来促进FD大鼠的胃动力。Objective To investigate the effects of Chaihushugansan decoction on the expression of endoplasmic reticulum stress-related molecules inositol-requiring enzyme 1（ IRE1） and TNF receptor associated factor 2（ TRAF2）in the antrum of rats with functional dyspepsia. Methods Thirty rats were randomly divided into the groups of Chaihushugansan,domperidone group,saline group,model group,normal control group. Rats in the normal control group were administrated with normal breeding,model group only clip tailwithout intragastric administration,while the others were gavaged with corresponding drugs. Life state of the rats were observed in the process of building. After 4 weeks of intervention,gastric emptying rate of rats were recorded. The mRNA level and protein level of IRE1,TRAF2 in the gastric antrum were detected with RT-PCR and immunohistochemistry respectively. Results The gastric emptying of model group,saline group decreased compared with that of normal control group（ P〈 0. 05）;the gastric emptying rate of domperidone groupand Chaihushugansan group increased significantly compared with that of model group（ P〈 0. 05）. The mRNA level of IRE1,TRAF2 and protein levels of IRE1,TRAF2 in model group and saline group significantly increased compared with that of normal control group; The mRNA level of IRE1,TRAF2 and protein levels of IRE1,TRAF2 in domperidone group,the group of Chaihushugansan decreased compared with that of model group. Conclusion Chaihushugansan can promote the functional dyspepsia rats stomach power,which may be related to the inhibition of endoplasmic reticulum stress-related molecules IRE1 and TRAF2.

关 键 词：柴胡疏肝散 功能性消化不良 内质网应激 IRE1 TRAF2 

分 类 号：R259[医药卫生—中西医结合]