C8gpm11抗菌肽对口内常见致病菌的作用研究  

Effect of antibacterial peptide C8gpm11 on oral common pathogenic bacteria

作　　者：侯梦瑶 孙应明[1,2] 营秀 张梦洁 陶睿 Hou Mengyao;Sun Yingming;YingXiu(Wuxi Clinical College of Anhui Medical University, Wuxi 214044;Dept of Stomatology,The 2LA 101th Hospital, Wuxi 214044;Dept of Stomatology,Bengbu MePical College,Bengbu 233000)

机构地区：[1]安徽医科大学无锡临床学院,无锡214044 [2]中国人民解放军第101医院口腔科,无锡214044 [3]蚌埠医学院口腔医学系,蚌埠233000

出　　处：《安徽医科大学学报》2018年第4期580-584,共5页Acta Universitatis Medicinalis Anhui

基　　金：无锡市卫生计生委科研项目(编号:Q201636);国家自然科学基金(编号:81300877)

摘　　要：目的研究新型抗菌肽C8gpm11对口内常见致病菌作用及机制。方法通过测定C8gpm11对口腔常见菌群的最小抑菌浓度(MIC)、最小杀菌浓度(MBC)、部分抑菌浓度(FIC)、部分杀菌浓度(FBC),以及测定唾液环境下C8gpm11的抑菌圈直径,分析其在口腔环境中的杀菌能力,利用扫描电镜观察细菌细胞膜形态。结果 C8gpm11对口内常见致病菌的抗菌作用呈现一定的浓度依赖性,唾液中C8gpm11活性略有影响。扫描电镜观察实验组细菌细胞膜表面有孔洞,细胞皱缩。C8gpm11与CHX联合使用具有协同抗菌作用。结论 C8gpm11具有针对口内常见致病菌的抑菌杀菌作用,为其临床应用提供了基础。Objective To study the antibacterial activity and mechanism of the novel antimicrobial peptide C8 gpm11 against oral common pathogenic microorganisms. Methods The microbicidal activity of C8 gpm11 was analyzed by MIC,MBC,FIC,FBC,and the bacteriostatic ring diameter of C8 gpm11 in the saliva environment.Scanning electron microscopy（ SEM） was used to observe the bacterial membrane. Results C8 gpm11 showed the microbicidal activity with a concentration-dependent manner. The bacteriostatic ability of C8 gpm11 in oral salivaryenvironment was not weakened obviously. C8 gpm11 in combination with CHX had a synergistic antimicrobial effect. Conclusion C8 gpm11 has a bactericidal effect against oral common pathogenic microorganisms,and provides a basis for its clinical value.

关 键 词：抗菌肽 C8gpm11 口内致病菌 

分 类 号：R781[医药卫生—口腔医学]