Measurement of cytoplasmic Ca^(2+) concentration in Saccharomyces cerevisiae induced by air cold plasma  

作　　者：董晓宇 

机构地区：[1]School of Life Science and Biotechnology, Dalian University

出　　处：《Plasma Science and Technology》2018年第4期2-7,共6页等离子体科学和技术（英文版）

基　　金：supported by National Natural Science Foundation of China (Grant Nos. 21246012, 21306015 and 21476032)

摘　　要：In this study, a novel approach to measure the absolute cytoplasmic Caconcentration([Ca]cyt) using the Caindicator fluo-3 AM was established. The parameters associated with the probe fluo-3 AM were optimized to accurately determine fluorescence intensity from the Ca-bound probe. Using three optimized parameters(final concentration of 6 m M probe,incubation time of 135 min, loading probe before plasma treatment), the maximum fluorescence intensity(F?=?527.8 a.u.) and the minimum fluorescence intensity(F?=?63.8 a.u.) were obtained in a saturated Casolution or a solution of lacking Ca. Correspondingly, the maximum [Ca]cytinduced by cold plasma was 1232.5 n M. Therefore, the Caindicator fluo-3 AM was successfully applied to measure the absolute [Ca]cytin Saccharomyces cerevisiae stimulated by cold plasma at atmospheric air pressure.In this study, a novel approach to measure the absolute cytoplasmic Ca^(2+) concentration([Ca^(2+) ]cyt) using the Ca^(2+) indicator fluo-3 AM was established. The parameters associated with the probe fluo-3 AM were optimized to accurately determine fluorescence intensity from the Ca^(2+) -bound probe. Using three optimized parameters(final concentration of 6 m M probe,incubation time of 135 min, loading probe before plasma treatment), the maximum fluorescence intensity(F_(max)?=?527.8 a.u.) and the minimum fluorescence intensity(F_(min)?=?63.8 a.u.) were obtained in a saturated Ca^(2+) solution or a solution of lacking Ca^(2+) . Correspondingly, the maximum [Ca^(2+) ]cytinduced by cold plasma was 1232.5 n M. Therefore, the Ca^(2+) indicator fluo-3 AM was successfully applied to measure the absolute [Ca^(2+) ]cytin Saccharomyces cerevisiae stimulated by cold plasma at atmospheric air pressure.

关 键 词：CYTOPLASMIC Ca2＋ concentration  SACCHAROMYCES cerevisiae  AIR cold plasma parameter optimization 

分 类 号：O539[理学—等离子体物理]