人牙髓干细胞成骨分化前后相关基因的表达分析  

Gene Expression of Human Dental Pulp Stem Cells during Osteogenic Differentiation

作　　者：林文珍 高丽[1,2] 牛晨光 胡戌琛[1,2] 苑克勇 马瑞 黄正蔚[1,2] LIN Wen--zhen;GAOLi;NIU Chen-- guang;HU Xu -- chen;YUAN Ke -- yong;MA Rui;HUANG Zheng -- wei(Department of Endodon- tics, Ninth People＇s Hospital, Shanghai J iaoTong University School of Medicin;Shanghai Key Laboratory of Stomatology ＆ Shanghai Research Institute of Stomatology, National Clinical Research Center of Stomatology, Shanghai 200011, China.)

机构地区：[1]上海交通大学医学院附属第九人民医院牙体牙髓病科 [2]上海市口腔医学重点实验室/上海市口腔医学研究所,国家口腔疾病临床研究中心,上海200011

出　　处：《口腔医学研究》2018年第4期375-379,共5页Journal of Oral Science Research

基　　金：国家自然科学基金(编号：81570964); 上海市教育委员会高峰高原学科建设计划

摘　　要：目的:分析人牙髓干细胞(hDPSCs)成骨诱导后相关基因的表达变化。方法:使用改良组织块法体外培养hDPSCs并检测其多向分化能力。hDPSCs成骨诱导后对其进行基因表达谱芯片技术和实时荧光定量PCR分析相关基因的表达情况。结果:hDPSCs能够进行成骨细胞和脂肪细胞分化。基因表达谱芯片技术分析得出hDPSCs在成骨诱导前后有1284个基因上调或下调2倍以上。其中,骨调节蛋白(osteomodulin,OMD)基因转录上调约50倍。实时荧光定量PCR显示OMD基因在成骨诱导过程中转录呈逐渐增加趋势。结论:hDPSCs成骨分化涉及多个基因调控。OMD基因也参与其中,其表达变化具有时间规律性,与成骨过程呈正相关。Objective：To evaluate the differences in gene expression of human dental pulp stem cells（hDPSCs）after osteogenetic induction..Methods：hDPSCs were cultured by explant method and the capability of multi-differentiation in hDPSCs was identified.The gene expressions were evaluated by microarray assay and quantitative realtime PCR after osteogenetic induction.Results：hDPSCs could differentiate into osteoblasts and adipocytes.The microarray assay revealed that the expression of 1284 genes were upregulated or downregulated more than twice after osteogenetic induction.Of these,the transcription of osteomodulin（OMD）gene was upregulated by fifty times.The results of qPCR indicated that the expression of OMD was gradually increased during osteogenetic induction.Conclusion：The osteogenetic differentiation of hDPSCs is regulated by multiple genes.OMD is one of these genes,and its expression is time-dependent and coincides with osteogenesis.

关 键 词：人牙髓干细胞 成骨分化 骨调节蛋白 

分 类 号：R78[医药卫生—口腔医学]