不同光质对茅苍术生长、抗氧化酶活性及挥发油含量的影响  

Effect of Different Light Quality on Growth,Anti-oxidative Enzyme Activities and Volatile Oil Content of Atractylodes lancea

作　　者：李强 姚霞[3] 孙楷 郭兰萍 杨全[1] 张燕 黄璐琦[1,2] LI Qiang;YAO Xia;SUN Kai;GUO Lan-ping;YANG Quan;ZHANG Yan;HUANG Lu-qi(School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China;State Key Laboratory of Dao-di Herbs Breeding Base, Joint Laboratory of Infinitus （China） Herbs Quality Research, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;China National of Traditional ＆ Herbal Medicine Co. Ltd. , Beijing 100195, China)

机构地区：[1]广东药科大学中药学院,广州510006 [2]中国中医科学院中药资源中心道地药材国家重点实验室培育基地、无限极中草药品质研究联合实验室,北京100700 [3]中国中药有限公司,北京100195

出　　处：《中国实验方剂学杂志》2018年第10期27-32,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81503194);中国中医科学院重点领域项目(ZZ10-27);国家质量基础的共性关键研究与应用重点专项(2016YFF0202802);国家重点研发计划项目(SQ2017YFC170295,2017YFC1700700)

摘　　要：目的：基于茅苍术在林下栽培优于露地栽培,开展不同光照试验,研究不同光质对茅苍术生长发育及挥发油成分含量的影响。方法：分别设置CK（空白组）,红光,蓝光,红光-蓝光（9∶1）[简称红蓝（9∶1）],红蓝（6∶1）,红蓝（3∶1）共6个不同光质组,生长9个月后采样分析。结果：红蓝（9∶1）光照条件下茅苍术的生物量（23.18 g）及4种挥发油成分（苍术素、苍术酮、苍术醇和β-桉叶醇）总量（3.74%）最高;其次是红蓝（6∶1）组,生物量18.32 g,4种成分总量2.97%。红蓝（9∶1）光照条件下净光合速率、蒸腾速率、气孔导度和胞间CO2浓度等光合参数均高于其他处理组,且大部分具有显著性差异。不同光质处理组下超氧化物歧化酶、过氧化物酶、还原型谷胱甘肽、还原型抗坏血酸和过氧化氢酶等抗氧化酶活性均高于空白组,且大部分具有显著性差异;红蓝（9∶1）光照下丙二醛含量显著低于空白组。结论：综合各项指标结果发现,红蓝（9∶1）光照条件最适合茅苍术的生长发育,为茅苍术产量及质量的提升提供新的种植模式。Objective：Based on the cultivation under the crown of Atractylodes lancea was better than the open field cultivation,different light experiments were carried out to investigate effect of different light qualities on the growth and the volatile oil content of A.lancea.Method：Six different light quality groups were set up,which included CK（blank group）,red light group,blue light group,red-blue（9∶1） group,red-blue（6∶1） group and red-blue（3∶1） group,9 months later,sampling analysis was carried out.Result：The biomass（23.18 g）and total content of four essential oil components（3.74%） of A.lancea were the highest under red-blue（9∶1）light conditions;followed by red-blue（6∶1）,the biomass was 18.32 g and the total content of four essential oil components was 2.97%.In addition,the net photosynthetic rate（Pn）,transpiration rate（Tr）,stomatal conductance（Gs） and intercellular CO2 concentration（Ci） of red-blue（9∶1） group were higher than those in other treatments,and most of them had significant differences.The activities of antioxidant enzyme [superoxide dismutase（SOD）,peroxidase（POD）,reduced glutathione（GSH）,reductive ascorbic acid（As A） and catalase（CAT） ]under different light quality groups were higher than those in the blank group,and most of them had significant differences.The malondialdehyde（MDA） content in red-blue（9∶1） group was significantly lower than in the blank group.Conclusion：The results show that red-blue（9∶1） illumination condition is most suitable for the growth and development of A.lancea,which provides new cropping patterns for improvement of yield and quality of this herb.

关 键 词：茅苍术 光质 生物量 挥发油 抗氧化酶 苍术醇 Β-桉叶醇 

分 类 号：R22[医药卫生—中医基础理论] R282[医药卫生—中医学]