夏枯草提取物对2型糖尿病ZDF大鼠肝糖原代谢的影响  

Effect of Prunellae Spica Extracts on Hepatic Glycogen in ZDF Rats

作　　者：田硕[1] 刘铜华 孙文[3] 吴丽丽[3] 秦灵灵[3] 侯毅[4] 董笑克 乐海波 洪明昭 TIAN Shuo;LIU Tong-hua;SUN Wen;WU Li-li;QIN Ling-ling;HOU Yi;DONG Xiao-ke;YUE Hai-bo;HONG Ming-zhao(First Clinical Medical College, Shaanxi University of Chinese Medicine, Xianyang 712000, China;Graduate School, Beijing University of Chinese Medicine, Beijing 100029, China;Health Cultivation Laboratory of the Ministry of Education, Beijing University of Chinese Medicine, Beijing 100029, China;Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China;Gttangming New District People＇s Hospital, Shenzhen 518106, China)

机构地区：[1]陕西中医药大学第一临床医学院,陕西咸阳712000 [2]北京中医药大学研究生院,北京100029 [3]北京中医药大学教育部中医养生学重点实验室,北京100029 [4]北京中医药大学东方医院,北京100078 [5]深圳市光明新区人民医院,广东深圳518106

出　　处：《中国实验方剂学杂志》2018年第10期101-106,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技支撑计划项目(2014BAI10B04)

摘　　要：目的：观察自发性2型糖尿病（T2DM）大鼠Zucker diabetic fatty（ZDF）（fa/fa）与ZDF（fa/＋）及夏枯草提取物干预后,ZDF（fa/fa）肝糖原含量及糖原合成酶（GSY2）,葡萄糖-6-磷酸酶（G-6-P）和葡萄糖转运蛋白2（GLUT2）基因表达的影响,探讨其调节肝糖原可能的机制。方法：以ZDF（fa/fa）大鼠16只为动物模型,分为模型组及夏枯草提取物组（12.25 mg·kg^-1·d^-1）,每组8只,另取ZDF瘦型大鼠8只为正常组。每2周测大鼠测空腹血糖（FBG）和体质量,第8周进行口服葡萄糖耐量试验（OGTT）,灌胃给药8周后腹主动脉取血,处死大鼠,留取肝脏组织-80℃储存及蜡块包埋备用。酶联免疫吸附法（ELISA）测定大鼠血清胰岛素（FINS）含量,过碘酸雪夫染色法（PAS染色法）检测肝糖原含量,实时荧光定量聚合酶链式反应（Real-time PCR）法检测GSY2,G-6-P和GLUT2 mRNA表达。结果：给药8周后,与模型组比较,夏枯草提取物明显增加大鼠肝糖原含量,降低大鼠体质量（P〈0.05,P〈0.01）和空腹血糖（P〈0.05,P〈0.01）,改善葡萄糖耐量（P〈0.05）,降低HOMA-IR指数（P〈0.01）,上调肝脏组织GSY2,GLU2 mRNA表达（P〈0.05）,下调G-6-P mRNA表达（P〈0.05）。结论：夏枯草提取物具有增加大鼠肝糖原含量的作用,且能降低大鼠体质量、血糖和胰岛素抵抗指数（HOMA-IR）指数。夏枯草提取物可能通过上调肝脏组织GSY2和GLU2 mRNA表达,下调G-6-P mRNA表达而发挥作用。Objective：To observe the effect of Prunellae Spica extracts on hepatic glycogen,and the gene expressions of glycogen synthase 2（GSY2）,glucose-6-phosphatase（G-6-P） and glucose transporter 2（GLUT2） in Zucker diabetic fatty rats（ZDF,fa/fa） and ZDF（fa/＋） with spontaneous type 2 diabetes.Method：Totally 16 male ZDF（fa/fa） rats were randomly divided into control group（Con） and treatment group（Prunellae Spica extracts,PS）.And 8 male ZDF（fa/＋） rats were used as normal group（Nor）.Treatment group were orally administered with PS（12.25 mg·kg^-1·d^-1） for 8 weeks.Fasting blood glucose（FBG） and body weight were measured every other week.Oral glucose tolerance test（OGTT） was tested in the 8 thweek.After 8 weeks,blood was taken from the abdominal aorta,and livers were collected and stored at-80 ℃.Fasting serum insulin（FINS） was tested by enzyme linked immunosorbent assay（ELISA）,hepatic glycogen amount was tested by Periodic Acid-Schiff stain（PAS stain）,GSY2,G-6-P and GLUT2 gene expressions were tested by Real-time PCR.Result：Compared with control group,the content of hepatic glycogen,HOMA-IR index and gene expressions of GSY2 and GLU2 were significantly increased（P〈0.05,P〈0.01）,while the body weight,blood glucose and gene expression of G-6-P were dramatically decreased in PS group（P〈0.05,P〈0.01）.Conclusion：Prunellae Spica extracts can increase the content of hepatic glycogen,and decrease body weight,blood glucose and HOMA-IR index in ZDF rats.Prunellae Spica extracts may play an important role in regulating the gene expressions of GSY2,GLU2 and G-6-P.

关 键 词：夏枯草提取物 ZUCKER DIABETIC fatty(ZDF) 2型糖尿病 肝糖原 胰岛素抵抗 

分 类 号：R22[医药卫生—中医基础理论] R2-031[医药卫生—中医学]