芹菜素对肥胖型小鼠脂肪组织AMPK信号通路的作用机制  

Effect and Mechanism of Apigenin on Fat Metabolism Disorder AMPK Signaling Way in Obese Mice

作　　者：黄链莎[1] 刘铜华[2] 孙文[2] 郭璇[2] 李迎真[1] 陈淑惠[1] 周鹏[1] HUANG Lian-sha;LIU Tong-hua;SUN Wen;GUO Xuan;LI Ying-zhen;CHEN Shu-hui;ZHOU Peng(Baoan District Hospital of Traditional Chinese Medicine, Shenzhen 518133, China;Key Laboratory of Health Cultivation of the Ministry of Education, Beijing University of Chinese Medicine, Beijing 100029, China)

机构地区：[1]深圳市宝安区中医院,广东深圳518133 [2]北京中医药大学教育部中医养生学重点实验室,北京100029

出　　处：《中国实验方剂学杂志》2018年第10期107-111,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广东省自然科学基金项目(2015A030310222)

摘　　要：目的：研究芹菜素对肥胖型db/db小鼠脂肪紊乱的作用及机制。方法：8周龄雄性db/db小鼠随机分为模型组、芹菜素组（50 mg·kg^-1·d^-1）,雄性C57BL/6J小鼠为正常组,药物干预4周后,检测小鼠体质量、空腹血糖（fasting blood glucose,FBG）,总胆固醇（total cholesterol,CHO）,甘油三酯（total triglyceride,TG）,游离脂肪酸（free fatty acids,FFA）,天冬氨酸氨基转移酶（aspartate aminotransferase,AST）,血肌酐（serum creatinine,SCr）,血尿素氮（blood urea nitrogen,BUN）;脂肪组织苏木素-伊红染色（hematoxylin-eosin staining,HE）观察脂肪细胞病理变化;实时荧光定量PCR（Real-time PCR）检测胆固醇应答元件结合蛋白1c（sterol regulatory element-binding protein 1c,SREBP1c）,脂肪酸合成酶（fatty acid synthetase,FAS）,过氧化物增殖物激活受体α（peroxisome proliferator activated receptorα,PPARα）基因表达;蛋白免疫印迹法（Western blot）检测腺苷酸活化蛋白激酶（phosphorylated adenosine monophosphate activated protein kinase,AMPK）和乙酰CoA羧化酶（acetyl-CoA carboxylase,ACC）磷酸化水平。结果：与模型组比较,芹菜素组小鼠体质量,FBG,CHO,TG,FFA明显降低（P〈0.05,P〈0.01）;HE染色脂肪细胞变小,脂质沉积减低;脂肪组织SREBP1c,FAS mRNA表达降低（P〈0.01）,p-AMPKα,p-ACC蛋白表达升高（P〈0.05,P〈0.01）。结论：芹菜素可以改善db/db小鼠脂肪代谢紊乱,可能是通过上调脂肪组织AMPK,ACC磷酸化水平,下调SREBP1c,FAS mRNA表达实现。Objective：To investigate the effect and mechanism of apigenin on fat metabolism disorder in Obese mice.Method：Eight-week-old male db/db mice were randomly divided into two groups,namely control group and apigenin group（50 mg·kg^-1·d^-1）,and 8 mice were used in each group.Another 8 male C57 BL/6 J mice were used as normal group.Body weight,serum glucose,lipid,histopathology and mRNA and protein expressions after a 4-week treatment were measured.Result：Compared with control group,apigenin group show significant decreases in body weight,fasting blood glucose（FBG）,total cholesterol（CHO）,total triglyceride（TG）,free fatty acids（FFA）,liver weight of mice（P〈0.05,P〈0.01）,and increases in fat deposition of adipose tissue according to the histological examination.The expressions of peroxisome proliferator activated receptor α（PPARα）,fatty acid synthetase（FAS） mRNA in adipose tissue of apigenin group decreased（P〈0.01）,and the protein expressions of p-phosphorylated adenosine monophosphate activated protein kinaseα（AMPKα） and p-acetyl-CoA carboxylase（ACC） were significantly increased（P〈0.01）.Conclusion：The effect of apigenin in improving fatty metabolism of db/db mice may be realized by up-regulating the phosphorylation of AMPK and ACC and down-regulating the expressions of SREBP1 c,FAS mRNA in adipose tissue.

关 键 词：芹菜素 脂肪代谢 腺苷酸活化蛋白激酶K/乙酰CoA羧化酶 胆固醇应答元件结合蛋白1c(SREBP1c) 脂肪酸合成酶(FAS) 过氧化物增殖物激活受体α(PPARα) 

分 类 号：R22[医药卫生—中医基础理论] R242[医药卫生—中医学]