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作 者:谢春芹[1] 凡军民[1] 许俊齐[1] 陈庶 曹正[1] XIE Chun-qin;FAN Jun-min;XU Jun-qi;CHEN Shu;CAO Zheng*(Department of Biological Engineering/Polytechnic College of Agriculture and Forestry, Jurong 212400, China)
机构地区:[1]江苏农林职业技术学院生物工程系,江苏句容212400
出 处:《山东农业大学学报(自然科学版)》2018年第3期477-483,共7页Journal of Shandong Agricultural University:Natural Science Edition
基 金:2015年江苏省"六大人才高峰"资助项目(2015-NY-024);江苏农林职业技术学院项目(2017KJ238);镇江市"1+1+N"新型农业技术项目(ZJNJ[2017]06)
摘 要:为了加快蝉花的产业化开发,在过去的3年里,主要研究了野生蝉花人工固体培养基栽培,将液体菌种接种到不同主料和不同氮源的培养基上,在相同的管理条件下进行培养,通过测定蝉花孢梗束的长速、鲜重、生物转化率,比较蝉花在不同培养基上的生长状况。结果表明,野生蝉花人工固体栽培的最适培养基为小麦40 g、氮源为蛋清2 g、营养液60 m L。In order to accelerate the industrialization development of Cordyceps cicadae, the cultivation of wild C. cicadae inartificial solid medium was studied in the past three years. The liquid strains were inoculated on different culture mediumwith different main materials and different nitrogen sources, under the same management conditions, the growth status ofcicada flower on different media was compared by measuring the growth speeds, fresh weight and biological transformationefficiencies of the flower sporophore growth. The results showed the optimum medium for artificial solid cultivation of wildcicada flower was wheat 40 g, nitrogen source 2 g, nutrient solution 60 mL.
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