乌头碱对肝癌MHCC97细胞生长、侵袭和迁移的调控作用及机制研究  

Regulatory effects and mechanism of aconitine on proliferation,invasion and migration of hepatoma carcinoma cell MHCC97

作　　者：熊慧生[1] 蒋参[1] 高瑞[1] 陈丽华 XIONG Hui-Sheng;JIANG Can;GAO Rui;CHEN Li-Hua(Department of Traditional Chinese Medicine, Chongqing Institute of Cancer Research, Chongqing 400030, Chin)

机构地区：[1]重庆市肿瘤研究所中医肿瘤科,重庆400030 [2]重庆市沙坪坝区覃家岗社区卫生服务中心中医科,重庆400038

出　　处：《中国免疫学杂志》2018年第5期688-692,共5页Chinese Journal of Immunology

基　　金：重庆市卫生和计生委中医药科技项目资助(No.ZY20150241)

摘　　要：目的:探究乌头碱对肝癌细胞生长、侵袭和迁移的作用机制。方法:MTT法检测不同浓度乌头碱对人肝癌细胞(MHCC97)增殖的影响;Transwell检测乌头碱对MHCC97细胞侵袭能力的影响;划痕实验检测乌头碱对MHCC97细胞迁移能力的影响;Western blot检测乌头碱对P38MAPK通路蛋白表达的影响。结果:根据预实验结果,选择5、10、20μg/ml三个浓度进行后续实验。乌头碱(10、20μg/ml)作用4 d后对MHCC96细胞增殖有明显的抑制作用,并能明显降低肝癌细胞的侵袭能力。乌头碱作用于MHCC97细胞后,细胞迁移能力明显减弱,p-P38/P38比值降低,p-MAPKAPK和p-HSP27表达明显减少。结论:乌头碱对肝癌细胞增殖、侵袭和迁移具有抑制作用,其作用机制可能与调控P38/MAPK信号通路激活有关。Objective： To investigate the anticancer activity and mechanism of aconitine on cell proliferation,invasion and migration of hepatoma carcinoma cell（ HCC）. Methods： The effect of aconitine at different concentrations on proliferation was calculated by MTT assay. The effects of aconitine on invasion and migration of HCC were measured by Transwell and wound healing assay. Western blot was employed to detect the protein levels of P38 MAPK signaling pathway-related proteins. Results： The concentrations of 5,10,20μg/ml were selected according to the results of pre-experiment. Aconitine（ 10,20 μg/ml） inhibits proliferation and invasion of MHCC97 cells markedly after cells were treated with aconitine for 4 days. Treatment with aconitine down-regulated the ability of migration and decreased the ratio of p-P38/P38 and protein levels of p-MAPKAPK and p-HSP27. Conclusion： Aconitine inhibits proliferation,invasion and migration,and the mechanism may related with P38 MAPK signaling pathway.

关 键 词：乌头碱 肝癌细胞 增殖 侵袭 迁移 

分 类 号：R735.7[医药卫生—肿瘤]