液相色谱-串联质谱法测定化妆品中新型防腐剂月桂酰精氨酸乙酯盐酸盐  

Determination of Ethyl Lauroyl Arginate Hydrochloride in Cosmetics by Liquid Chromatography-Tandem Mass Spectrometry

作　　者：刘丁[1] 杨晋青[1] 赵艳菊[1] 彭亚锋[1] LIU Ding,YANG Jin-qing,ZHAO Yan-ju,PENG Ya-feng(Shanghai Institute of Quality Inspection and Technical Research, Shanghai 200233, Chin)

机构地区：[1]上海市质量监督检验技术研究院,上海200233

出　　处：《香料香精化妆品》2018年第2期53-58,共6页Flavour Fragrance Cosmetics

基　　金：基于出口化妆品中月桂酰精氨酸乙酯盐酸盐测定技术研究(项目编号17TBT008)

摘　　要：建立了液相色谱-串联质谱法测定化妆品中防腐剂月桂酰精氨酸乙酯盐酸盐(ethyl lauroyl arginate hydrochloride,以下简称LAE)的分析方法。采用超声波溶剂提取法提取样品中的LAE,经筛选确定使用Waters ACQUITY UPLC~? BEH C_(18)(2.1 mm×100 mm×1.7μm)色谱柱,流动相为质量分数0.1%甲酸水溶液和乙腈,梯度洗脱,然后用质谱法检测,以外标法定量。最佳提取条件为:提取溶剂甲醇,提取时间20 min,提取次数3次。LAE的线性范围为0.5～10 ng/m L,线性相关系数为0.9990。方法的检出限为1μg/kg,定量限为10μg/kg;通过加标回收率试验,确定方法的加标回收率范围为84.9%~110.0%。该方法简单、便捷,适用于化妆品中LAE含量的测定。A method for detection of ethyl lauroyl arginate hydrochloride（LAE） in cosmetics by liquid chromatography-tandem mass spectrometry（LC-MS/MS） was established. LAE was obtained by ultrasonic extraction from samples, separated by an ACQUITY UPLC BEH C18（2.1 mm×100 mm×1.7 μm） column using 0.1%（mass ratio）formic acid water solution-acetonitrile as mobile phase for gradient elution and then detected by tandem mass spectrometry with external standard method for quantification. The optimum extracting conditions were as follows： methanol was used as solvent, extracting time was 20 min, and extraction frequency was 3 times. LAE had a good linearity in the range of 0.5 to 10 ng/m L（r= 0.9992）. The limit of detection was 1.0 μg/kg and the limit of quantification was 10.0 μg/kg. The recovery rates were ranged from 84.9% to 110.0%. This method is simple and convenient, which is suitable for the detection of LAE in cosmetics.

关 键 词：月桂酰精氨酸乙酯盐酸盐 液相色谱-串联质谱法 化妆品 

分 类 号：O657.63[理学—分析化学] TQ658[理学—化学]