基于线粒体16S rRNA、COX1和Cytb基因探讨11种小丑鱼的系统发育关系  

Molecular Phylogeny Determined by Mitochondrial Genes 16S rRNA,COX1 and Cytb of Eleven Anemonefish Species

作　　者：何丽斌[1] 陈芳 朱志煌 马敏宁 张河长 吴孙龙 周宸[1] HE Li-bin;CHEN Fang;ZHU Zhi-huang;MA Min-ning;ZHANG He-chang;WU Sun-long;ZHOU Chen(Fujian Fisheries Research Institute, Xiarnen, Fujian 361013, China;Fujian Coral Sea Ecological Technology Co. Ltd. , Fuzhou, Fujian 350102, China)

机构地区：[1]福建省水产研究所,福建厦门361013 [2]福建珊瑚海生态科技有限公司,福建福州350102

出　　处：《福建农业学报》2018年第3期230-235,共6页Fujian Journal of Agricultural Sciences

基　　金：福建省海洋与渔业结构调整专项[闽财指(2016)841号];福建省科技计划项目--省属公益类科研院所基本科研专项(2013R001-7);福州市"十三五"海洋经济创新发展示范项目(FZHJ11);厦门市科学技术局科技计划专项(3502Z20142008)

摘　　要：采用线粒体16S rRNA、COX1和Cytb基因测定11种小丑鱼的序列,分析其基因序列差异和遗传距离关系,并用邻接法、最大似然法和贝叶斯法构建系统进化树,探讨了11种小丑鱼的系统发育关系。序列分析结果显示,经比对处理后的3个基因总长度4 388bp,其中多态位点1 028个,简约信息位点616个,转换与颠换比为2.12,A+T的平均含量为54.7%,高于C+G的平均含量45.3%。系统发育关系表明:(1)双锯鱼亚科为单系发生,但双锯鱼属不为单系发生,建议对棘颊雀鲷属重新定位;在双锯鱼属中,公子小丑鱼Amphiprion ocellaris和黑边公子小丑鱼Amphiprion percula聚为一支,为双锯鱼亚科分子亲缘关系基础;(2)透红小丑鱼Premnas biaculeatus在双锯鱼属的定位较为模糊,贝叶斯树显示透红小丑鱼P.biaculeatus与公子小丑鱼A.ocellaris和黑边公子小丑鱼A.percula聚成一支并位于该支的底端,而NJ树和ML树中透红小丑鱼却没能和公子小丑鱼A.ocellaris和黑边公子小丑鱼A.percula聚为一支,而是和其他小丑鱼汇聚成一支;(3)透红小丑鱼P.biaculeatus与金透红小丑鱼Premnas epigrammata是同一种。Sequences of mitochondrial 16 S rRNA,COX1 and Cytb genes were determined to establish the phylogenetic relationship among 11 species of anemonefish using the neighbor-joining（NJ）,maximum-likelihood（ML）and Bayesian methods with the divergence and genetic distances determined as well.The 3 genes had a combined length of 4 388 bp that consisted of 1 028 polymorphic and 616 parsimonious-information sites.The ratio of transition to transversion was 2.12,and the average A＋T content was 54.7%,which was higher than 45.3%for C＋ G in the genes.It was found that（1）the subfamily Amphiprioninae was monophyly,but the genus Amphiprion was not monophyletic,implying Premnas should be reclassified;（2）the phylogenetic trees grouped the percula complex into a clade that formed the ancestral taxon of anemonefish,but the phylogenetic relationship was uncertain in regard to Premnas biaculeatus;and,while the Bayesian tree placed the percula complex and P.biaculeatus in a same clade,NJ and ML tree classified P.biaculeatus in a clade with subgenera Amphiprion,Paramphiprion and Phalerebus;and,（3）the genetic distance between species suggested a possibility of P.biaculeatus and Premnas epigrammata being identical.

关 键 词：16S RRNA基因 COX1基因 CYTB基因 小丑鱼 遗传差异 系统进化 

分 类 号：S917.4[农业科学—水产科学]